Glyburide inhibits the bone resorption induced by traumatic occlusion in rats
Glyburide inhibits the bone resorption induced by traumatic occlusion in rats
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DOI：
10.1111/jre.12731
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发表时间：
2020-06-01
影响因子：
3.5
通讯作者：
Sakagami, Ryuji
中科院分区：
文献类型：
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作者：
Arita, Yoichi;Yoshinaga, Yasunori;Sakagami, Ryuji
目的研究格列本脲是否能抑制咬合创伤引起的骨破坏，如咬合创伤，过度的机械应力可诱导IL-1 β的表达，并可能参与骨吸收。NLRP 3炎性小体与IL-1 β表达有关，但目前尚不清楚NLRP 3炎性小体抑制剂格列本脲是否能抑制大鼠咬合创伤。创伤组在上颌右第一磨牙的咬合面上附加金属丝，对下颌右第一磨牙施加咬合创伤。在创伤+格列本脲组中，从咬合创伤诱导前1天开始每24小时口服施用NLRP 3抑制剂格列本脲。5或10天后处死大鼠，取上颌第一磨牙和邻近组织进行组织病理学研究。结果第5天，创伤组骨吸收明显高于对照组和创伤+格列本脲组，创伤组破骨细胞和IL-1 β、NLRP 3和RANKL阳性细胞数量明显高于对照组和创伤+格列本脲组。格列本脲抑制大鼠创伤性咬合引起的骨吸收提示NLRP 3/IL-1 β通路可能与创伤性咬合诱导的骨吸收有关。
Objective To examine whether glyburide inhibits bone destruction caused by traumatic occlusion in a rat occlusal trauma model.Background Excessive mechanical stress, such as traumatic occlusion, induces expression of IL-1 beta and may be involved in bone resorption. NLRP3 inflammasomes have been linked to IL-1 beta expression, but it is currently unclear whether glyburide, the inhibiter of NLRP3 inflammasome, suppresses occlusal trauma in rats.Methods Male SD rats aged 7 weeks were used. In the trauma group, the occlusal surface of the maxillary first right molar was raised by attaching a metal wire to apply occlusal trauma to the mandibular first right molar. In the trauma + glyburide group, the NLRP3 inhibitor glyburide was administered orally every 24 hours from 1 day before induction of occlusal trauma. Rats were euthanized after 5 or 10 days, and the maxillary first molars were harvested with the adjacent tissues for histopathological investigation. Immunohistochemical expression of IL-1 beta, NLRP3, and RANKL was also assessed.Results On day 5, bone resorption was significantly greater in the trauma group compared with the control group or the trauma + glyburide group, and there were significantly higher numbers of osteoclasts and cells positive for IL-1 beta, NLRP3, and RANKL in the trauma group.Conclusion In this study, glyburide inhibits bone resorption by traumatic occlusion in rats. It suggests that the NLRP3/IL-1 beta pathway might be associated with bone resorption induced by traumatic occlusion.