Assessing Microglial Dynamics by Live Imaging.
Assessing Microglial Dynamics by Live Imaging.
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DOI：
10.3389/fimmu.2021.617564
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发表时间：
2021
影响因子：
7.3
通讯作者：
Koyama R
中科院分区：
文献类型：
--
作者：
Andoh M;Koyama R
小胶质细胞在大脑中具有高度动态性，具有在个体生命过程中迁移、增殖和吞噬的能力。实时成像是检查小胶质细胞行为如何调节及其如何影响周围环境的有用工具。然而，小胶质细胞对环境刺激敏感，因此它们可能在体内实时成像过程中改变其状态，这主要是由于手术损伤，而在体外则可能由于与培养条件相关的各种影响而改变。因此，在不损害生理条件下小胶质细胞特性的情况下很难进行实时成像。为了克服这一障碍，开发了各种实验条件；最近，尽管存在各种限制，但在体内、离体和体外对所谓的监视小胶质细胞进行实时成像已成为可能。现在，我们可以根据研究目的选择体内、离体或体外活体成像系统。在这篇综述中，我们讨论了每个实验系统的优点和缺点，并概述了通过实时成像阐明的小胶质细胞行为的生理意义和分子机制。
Microglia are highly dynamic in the brain in terms of their ability to migrate, proliferate, and phagocytose over the course of an individual's life. Real-time imaging is a useful tool to examine how microglial behavior is regulated and how it affects the surrounding environment. However, microglia are sensitive to environmental stimuli, so they possibly change their state during live imaging in vivo, mainly due to surgical damage, and in vitro due to various effects associated with culture conditions. Therefore, it is difficult to perform live imaging without compromising the properties of the microglia under physiological conditions. To overcome this barrier, various experimental conditions have been developed; recently, it has become possible to perform live imaging of so-called surveillant microglia in vivo, ex vivo, and in vitro, although there are various limitations. Now, we can choose in vivo, ex vivo, or in vitro live imaging systems according to the research objective. In this review, we discuss the advantages and disadvantages of each experimental system and outline the physiological significance and molecular mechanisms of microglial behavior that have been elucidated by live imaging.
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耦合的增殖和凋亡维持成人大脑中小胶质细胞的快速离职。
影响因子：
8.8
作者：
Askew K;Li K;Olmos-Alonso A;Garcia-Moreno F;Liang Y;Richardson P;Tipton T;Chapman MA;Riecken K;Beccari S;Sierra A;Molnár Z;Cragg MS;Garaschuk O;Perry VH;Gomez-Nicola D
通讯作者：
Gomez-Nicola D
影响因子：
4.6
作者：
Brawek B;Liang Y;Savitska D;Li K;Fomin-Thunemann N;Kovalchuk Y;Zirdum E;Jakobsson J;Garaschuk O
通讯作者：
Garaschuk O
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
6.2
作者：
Eyo, Ukpong;Dailey, Michael E.
通讯作者：
Dailey, Michael E.
影响因子：
5.3
作者：
Carbonell, WS;Murase, SI;Mandell, JW
通讯作者：
Mandell, JW