The cells of the rabbit meniscus: their arrangement, interrelationship, morphological variations and cytoarchitecture
The cells of the rabbit meniscus: their arrangement, interrelationship, morphological variations and cytoarchitecture
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DOI：
10.1046/j.1469-7580.2000.19850525.x
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发表时间：
2001-05-01
影响因子：
2.4
通讯作者：
Rattner, JB
中科院分区：
文献类型：
--
作者：
Le Graverand, MPH;Ou, Y;Rattner, JB
使用细胞骨架蛋白抗体在成年兔半月板内鉴定出四种主要形态上不同的细胞类别。半月板的纤维软骨区域存在两类细胞。这些半月板细胞表现出从细胞体延伸出来的长细胞突起。在半月板内部透明样区域发现的第三种细胞类型呈圆形且缺乏突起。沿着半月板的表面区域发现了第四种具有梭形形状且没有细胞质突出的细胞类型。使用连接蛋白 43 的单克隆抗体，在纤维软骨区域观察到大量间隙连接，而在半月板的透明样或浅层区域的细胞中却没有观察到任何间隙连接。半月板内的大多数细胞表现出其他特定特征，例如初级纤毛和 2 个中心体。半月板细胞亚型的位置及其形态和结构支持这样的假设，即它们的特定特征是半月板响应不同类型的环境机械载荷的能力的基础。
Four major morphologically distinct classes of cells were identified within the adult rabbit meniscus using antibodies to cytoskeletal proteins. Two classes of cell were present in the fibrocartilage region of the meniscus. These meniscal cells exhibited long cellular processes that extended from the cell body. A third cell type found in the inner hyaline-like region of the meniscus had a rounded form and lacked projections. A fourth cell type with a fusiform shape and no cytoplasmic projections was found along the superficial regions of the meniscus. Using a monoclonal antibody to connexin 43, numerous gap junctions were observed in the fibrocartilage region, whereas none were seen in cells either from the hyaline-like or the superficial zones of the meniscus. The majority of the cells within the meniscus exhibited other specific features such as primary cilia and 2 centrosomes. The placement of the meniscal cell subtypes as well as their morphology and architecture support the supposition that their specific characteristics underlie the ability of the meniscus to respond to different types of environmental mechanical loads.