Potential of antibody test using Schistosoma mansoni recombinant serpin and RP26 to detect light-intensity infections in endemic areas.
Potential of antibody test using Schistosoma mansoni recombinant serpin and RP26 to detect light-intensity infections in endemic areas.
复制标题
使用曼氏血吸虫重组丝氨酸蛋白酶抑制剂和 RP26 进行抗体测试检测流行地区光强度感染的潜力。
DOI：
10.1016/j.parint.2021.102346
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发表时间：
2021
影响因子：
1.9
通讯作者：
Hamano S
中科院分区：
文献类型：
--
作者：
Tanaka M;Kildemoes AO;Chadeka EA;Cheruiyot BN;Sassa M;Moriyasu T;Nakamura R;Kikuchi M;Fujii Y;de Dood CJ;Corstjens PLAM;Kaneko S;Maruyama H;Njenga SM;de Vrueh R;Hokke CH;Hamano S
血吸虫病仍然是一个世界性的公共卫生问题，特别是在撒哈拉以南非洲。世界卫生组织在2030年被忽视的热带病（NTDs）路线图中将其作为公共卫生问题消除的目标。要实现这些目标，就必须采取协调一致的行动，灵活应对挑战。更好的诊断测试可以通过监测疾病趋势和评估干预措施的有效性来加速消除进展;然而，目前的检查，如Kato-Katz技术，在检测光强度感染方面的能力有限。与参考标准Kato-Katz技术相比，床旁循环阴极抗原（POC-CCA）检测显示出更高的灵敏度，但在低感染强度下仍缺乏足够的灵敏度。在这项研究中，我们研究了重组蛋白抗原的抗体反应，曼氏血吸虫丝氨酸蛋白酶抑制剂（SmSerpin）和RP 26，通过酶联免疫吸附试验（ELISA）在血浆样品中的光强度感染。用重组SmSerpin和RP 26的混合抗原检测敏感性为83.7%。用S. SmSEA的特异性为29.7%，而混合抗原的特异性为61.4%。我们认为，SmSerpin和RP 26蛋白抗原的抗体检测是有效的检测。mansonlight强度infections感染.我们的研究表明，检测重组蛋白抗原的抗体监测血吸虫病在低流行背景下的传播的潜力。
Schistosomiasis remains a worldwide public health problem, especially in sub-Saharan Africa. The World Health Organization targets the goal for its elimination as a public health problem in the 2030 Neglected Tropical Diseases (NTDs) Roadmap. Concerted action and agile responses to challenges will be necessary to achieve the targets. Better diagnostic tests can accelerate progress towards the elimination by monitoring disease trends and evaluating the effectiveness of interventions; however, current examinations such as Kato–Katz technique are of limited power to detect light-intensity infections. The point-of-care circulating cathodic antigen (POC-CCA) test shows a higher sensitivity compared to the reference standard, Kato-Katz technique, but it still lacks sufficient sensitivity with low infection intensity. In this study, we examined antibody reactions against recombinant protein antigens; Schistosoma mansoni serine protease-inhibitor (SmSerpin) and RP26, by enzyme-linked immunosorbent assay (ELISA) in plasma samples with light-intensity infection. The sensitivity using the cocktail antigen of recombinant SmSerpin and RP26 showed 83.7%. The sensitivity usingS. mansonisoluble egg antigen (SmSEA) was 90.8%, but it showed poor specificity (29.7%), while the cocktail antigen presented improved specificity (61.4%). We conclude that antibody detection to the SmSerpin and RP26 protein antigens is effective to detectS. mansonilight-intensity infections. Our study indicates the potential of detecting antibody against recombinant protein antigens to monitor the transmission of schistosomiasis in low endemicity contexts.