Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision- and ozone-induced dissociation
Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision- and ozone-induced dissociation
复制标题
DOI：
10.1255/ejms.1300
复制
发表时间：
2015-01-01
影响因子：
1.3
通讯作者：
Blanksby, Stephen J.
中科院分区：
文献类型：
--
作者：
Kozlowski, Rachel L.;Mitchell, Todd W.;Blanksby, Stephen J.
密切相关的脂质异构体的区分对于我们不断发展的脂质生物化学理解越来越重要，但它对当代色谱和质谱分析同样具有挑战性。最近，我们描述了一种基于碰撞与臭氧诱导解离（CID/OzID）相结合的新型离子激活方法，用于识别甘油磷脂中的相对酰基链取代位置。在这里，我们首次证明，CID/OzID 可以有效地与反相色谱结合，以实现磷脂酰胆碱区域异构体对的分离和明确鉴定，这些磷脂酰胆碱区域异构体对仅在甘油主链上酰基链的排列上有所不同。
The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision-with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.