New method for affinity adsorbent screening using gold colloids.
New method for affinity adsorbent screening using gold colloids.
复制标题
DOI：
10.1021/la2017853
复制
发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Jun Wang;Wei Wang;Q. Wei;Saihui Zhang;Zhi Yuan
中科院分区：
文献类型：
--
作者：
Jun Wang;Wei Wang;Q. Wei;Saihui Zhang;Zhi Yuan
利用胶体金独特的光学性质，发展了一种快速、有效的吸附剂配体筛选方法。吸附物和配体之间的不同相互作用诱导不同状态的聚集的金胶体，和相关的不同的颜色变化的胶体已被用来估计的亲和力的配体对吸附物。在这项工作中，磷酸化肽CGGFGGpSG附加到金胶体，以获得吸附物修饰的金胶体（CG 8-AuNPs）。候选配体Dpa-Zn（2+）、DMAPAA和AAn与丙烯酰胺共聚以形成线性聚合物和交联的CG 8-AuNPs以诱导聚集。筛选结果表明，Dpa-Zn（2+）在10 μM浓度下对金胶体的亲和性最强，其诱导金胶体变色的能力远低于配体DMAPAA（40 μM）和AAn（几乎没有变色）。随后的统计吸附实验证实了这些筛选结果，吸附剂A-AAm-Dpa-Zn（2+）显示出对CG-8的最高吸附容量（426 mg/g），几乎是吸附剂A-AAm-DMAPAA的两倍。该报道的方法具有低样品消耗，并且可以通过肉眼简单地监测筛选。
A rapid, effective method for the screening of adsorbent ligands based on the unique optical properties of gold colloid has been developed. Different interactions between adsorbate and ligands induce different states of aggregation of the gold colloid, and the associated distinct color changes of the colloid have been utilized to estimate the affinity of the ligands toward the adsorbate. In this work, phosphorylated peptide CGGFGGpSG was appended to a gold colloid to obtain the adsorbate-modified gold colloid (CG8-AuNPs). Candidate ligands Dpa-Zn(2+), DMAPAA, and AAn were copolymerized with acrylamide to form linear polymers and cross-linked CG8-AuNPs to induce aggregation. Screening of the candidate ligands revealed that Dpa-Zn(2+) showed the highest affinity among those tested, inducing a color change of the gold colloid at a concentration of 10 μM, which is much lower than those of ligands DMAPAA (40 μM) and AAn (almost no color change could be observed). Subsequent statistical adsorption experiments confirmed these screening results, with the adsorbent A-AAm-Dpa-Zn(2+) showing the highest adsorption capacity (426 mg/g) for CG-8, almost twice that of adsorbent A-AAm-DMAPAA. This reported method has low sample consumption, and the screening may be simply monitored by the naked eye.