Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis
Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis
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DOI：
10.1002/1873-3468.12659
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发表时间：
2017-05-01
期刊：
影响因子：
3.5
通讯作者：
Ishikawa, Takahiro
中科院分区：
文献类型：
--
作者：
Tanaka, Yuji;Ogawa, Takahisa;Ishikawa, Takahiro
植物鞭毛虫细小裸藻产生大量的裸藻淀粉 (PM)，这是一种线性 β-1,3-葡聚糖链的聚集体，作为储存多糖。 PM是由尿苷二磷酸-葡萄糖合成的，但其形成机制尚不清楚。先前在眼虫转录组分析中将类葡聚糖合酶 (EgGSL) 1 和 EgGSL2 两种酶确定为 PM 合成的候选酶。在这里，我们对这些酶进行了反向遗传分析。敲低 EgGSL2（而非 EgGSL1）可显着抑制眼虫细胞中 PM 的积累。此外，在从眼虫细胞中提取的 PM 相关膜组分中检测到了 β-1,3-葡聚糖的合成。我们的研究结果表明 EgGSL2 是 PM 生物合成的主要酶。
The phytoflagellate Euglena gracilis produces a large amount of paramylon (PM), a conglomerate of liner beta-1,3-glucan chains, as a storage polysaccharide. PM is synthesized from uridine diphosphate-glucose, but its mechanism of formation is largely unknown. Two enzymes, glucan synthase-like (EgGSL) 1 and EgGSL2 were previously identified as candidates for PM synthesis in a Euglena transcriptome analysis. Here, we performed a reverse genetic analysis on these enzymes. Knockdown of EgGSL2, but not EgGSL1, significantly inhibits PM accumulation in Euglena cells. Additionally, b-1,3-glucan synthesis is detected in a PM-associated membrane fraction extracted from Euglena cells. Our findings indicate that EgGSL2 is the predominant enzyme for PM biosynthesis.