Complete coding sequences of the rabbitpox virus genome
Complete coding sequences of the rabbitpox virus genome
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DOI：
10.1099/vir.0.81331-0
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发表时间：
2005-11-01
影响因子：
3.8
通讯作者：
Upton, C
中科院分区：
文献类型：
--
作者：
Li, G;Chen, N;Upton, C
兔痘病毒（RPXV）是一种对家兔具有高度致病性的病毒，长期以来一直被怀疑是牛痘病毒的近亲。为了探索这些问题，对兔痘病毒基因组的完整编码区进行了测序，以便与牛痘病毒和其他正痘病毒的测序株进行比较。RPXV Utrecht株（RPXV-UTR）基因组全长197731个核苷酸，不包括两端的发夹结构。RPXV-UTR基因组具有66.5%的A+T含量，184个推定的功能基因和12个在其他正痘病毒中完整的片段化ORF区域。RPXV-UTR基因组的序列揭示了两个RPXV-UTR基因在天花病毒（VARV;天花的病原体）中具有直向同源物，但在牛痘病毒（VACV）株中没有。这些基因是锌环指蛋白基因（RPXV-UTR-008）和锚蛋白重复家族蛋白基因（RPXV-UTR-180）。第三个基因编码趋化因子结合蛋白（RPXV-UTR-001/184），在VARV中是完整的，但仅在一些VACV株中起作用。RPXV和其他正痘病毒之间的进化关系的检查进行了使用的中央143 kb的DNA序列之间的所有完全测序的正痘病毒和49个基因产物中存在的所有完全测序的脊索痘病毒的蛋白质序列保守。这些分析的结果都证实了RPXV-UTR与VACV最密切相关，并表明RPXV没有直接从任何测序的VACV毒株进化而来，因为RPXV含有先前在任何VACV中未鉴定出的719 bp区域。
Rabbitpox virus (RPXV) is highly virulent for rabbits and it has long been suspected to be a close relative of vaccinia virus. To explore these questions, the complete coding region of the rabbitpox virus genome was sequenced to permit comparison with sequenced strains of vaccinia virus and other orthopoxviruses. The genome of RPXV strain Utrecht (RPXV-UTR) is 197 731 nucleotides long, excluding the terminal hairpin structures at each end of the genome. The RPXV-UTR genome has 66.5 % A+T content, 184 putative functional genes and 12 fragmented ORF regions that are intact in other orthopoxviruses. The sequence of the RPXV-UTR genome reveals that two RPXV-UTR genes have orthologues in variola virus (VARV; the causative agent of smallpox), but not in vaccinia virus (VACV) strains. These genes are a zinc RING finger protein gene (RPXV-UTR-008) and an ankyrin repeat family protein gene (RPXV-UTR-180). A third gene, encoding a chemokine-binding protein (RPXV-UTR-001/184), is complete in VARV but functional only in some VACV strains. Examination of the evolutionary relationship between RPXV and other orthopoxviruses was carried out using the central 143 kb DNA sequence conserved among all completely sequenced orthopoxviruses and also the protein sequences of 49 gene products present in all completely sequenced chordopoxviruses. The results of these analyses both confirm that RPXV-UTR is most closely related to VACV and suggest that RPXV has not evolved directly from any of the sequenced VACV strains, since RPXV contains a 719 bp region not previously identified in any VACV.