Allotetraploidy of Zoysia species with 2n=40 based on a RFLP genetic map
Allotetraploidy of Zoysia species with 2n=40 based on a RFLP genetic map
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DOI：
10.1007/s001220051131
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发表时间：
1999-04
影响因子：
5.4
通讯作者：
M. Yaneshita;S. Kaneko;T. Sasakuma
中科院分区：
文献类型：
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作者：
M. Yaneshita;S. Kaneko;T. Sasakuma
结缕草属植物RFLP连锁图谱。（2n=40）是一种暖季草坪草，利用种间杂交种的自花授粉后代构建。115个DNA克隆中，55个基因组克隆、38个cDNA克隆和7个光合酶基因克隆存在等位基因- rflp带带，占比87.0%。我们发现有26个探针在自花授粉后代中独立检测到两个或两个以上的位点分离。结缕草的RFLP连锁图谱。由22个连锁群中的115个位点组成，大小从12.5 cM到141.3 cM不等，总图谱距离为1506 cM。6个RFLP位点（5.4%）存在显著的分离畸变（P<0.01）。6个基因座中的2个被映射到连锁组II，另外2个基因座被映射到连锁组VII。在结缕草的RFLP连锁图谱中，鉴定出5对共享一系列重复位点的连锁群，它们的顺序大致相同。因此，我们得出结论：zoysiaspp。与2n=40应被认为是异体四倍体，它可能是从祖先进化的基本染色体数目为10 （x=10）。
A RFLP linkage map ofZoysiaspp. (2n=40), a warm-season turfgrass, was constructed by using the self-pollinated progenies obtained from an interspecific hybrid. Out of 115 DNA clones tested, 100 (87.0%), including 55 genomic clones, 38 cDNA clones, and seven gene clones encoding photosynthetic enzymes showed allelic-RFLP banding patterns among the parental accessions. We found that 26 probes detected two or more loci segregating in the self-pollinated progenies independently. The RFLP linkage map ofZoysiaspp. consists of 115 loci in 22 linkage groups ranging in size from 12.5 cM to 141.3 cM with a total map distance of 1506 cM. Six RFLP loci (5.4%) showed significant segregation distortion (P<0.01). Two loci out of six were mapped to linkage group II, and another two loci were mapped to group VII. In the RFLP linkage map of zoysiagrass, five pairs of linkage groups sharing a series of duplicated loci with approximately the same order were identified. Therefore, we conclude thatZoysiaspp. with 2n=40 should be considered as allotetraploids, which might have evolved from progenitors with a basic chromosome number of ten (x=10).