An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers
An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers
复制标题
DOI：
10.1007/s11046-010-9284-7
复制
发表时间：
2010-06-01
期刊：
影响因子：
5.5
通讯作者：
Johnson, Elizabeth M.
中科院分区：
文献类型：
--
作者：
Borman, Andrew M.;Fraser, Mark;Johnson, Elizabeth M.
在这里，我们提出了一个显着改进的版本，该方法是我们之前发布的使用 Whatman FTA(R) 滤纸基质技术从纯培养物中提取真菌基因组 DNA 的方法。这种修改后的方案速度非常快，比我们原来的方法显着更具成本效益，而且重要的是，在采用 FTA 技术时，大大减少了顺序过滤器之间潜在的交叉污染问题。
Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA(R) filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.