Controlling and exploiting cell-to-cell variation in metabolic engineering
Controlling and exploiting cell-to-cell variation in metabolic engineering
复制标题
控制和利用代谢工程中的细胞间变异
DOI：
10.1016/j.copbio.2018.08.013
复制
发表时间：
2019
影响因子：
7.7
通讯作者：
Dunlop, Mary J
中科院分区：
文献类型：
--
作者：
Wang, Tiebin;Dunlop, Mary J
细胞间变异对代谢工程有着重要的影响。控制甚至利用变异的策略可以提高产量。量化变异的新技术将使创新方法成为可能。由于局部环境、遗传变异和基因随机表达的差异，群体中的单个细胞可以表现出不同的表型。了解这种细胞间变异对于代谢工程应用非常重要，因为变异性会影响生产。例如，最近的研究表明，工程细胞之间的生产可能是高度异质性的，管理这种多样性的策略可以提高生物合成产品的产量。这些结果表明控制变异作为改善工程化细胞性能的新方法的潜力。在这篇综述中，我们专注于确定细胞间变异在代谢工程应用中的起源，并讨论了可用于减少、接受甚至利用细胞间变异的策略的最新进展。
HighlightsCell-to-cell variation can have a significant impact on metabolic engineering.Strategies for controlling or even exploiting variation can improve production.New technologies for quantifying variation will enable innovative approaches.Individual cells within a population can display diverse phenotypes due to differences in their local environment, genetic variation, and stochastic expression of genes. Understanding this cell-to-cell variation is important for metabolic engineering applications because variability can impact production. For instance, recent studies have shown that production can be highly heterogeneous among engineered cells, and strategies that manage this diversity improve yields of biosynthetic products. These results suggest the potential of controlling variation as a novel approach towards improving performance of engineered cells. In this review, we focus on identifying the origins of cell-to-cell variation in metabolic engineering applications and discuss recent developments on strategies that can be employed to diminish, accept, or even exploit cell-to-cell variation.