Genetic factors governing bacterial virulence and host plant susceptibility during Agrobacterium infection.
Genetic factors governing bacterial virulence and host plant susceptibility during Agrobacterium infection.
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DOI：
10.1016/bs.adgen.2022.08.001
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发表时间：
2022
影响因子：
--
通讯作者：
Citovsky, Vitaly
中科院分区：
文献类型：
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作者：
Lacroix, Benoit;Citovsky, Vitaly
农杆菌属的一些物种代表了独特的细菌病原体，它们能够通过在宿主基因组中转移和整合自己的DNA片段（T-DNA，转移的DNA）来遗传地改造植物。而在自然界中，这一过程导致受感染的植物细胞（肿瘤）不受控制的生长，农杆菌的这种能力已被广泛用作产生转基因植物的关键工具，用于研究和生物技术。农杆菌的毒力依赖于一系列毒力基因，这些基因大多编码在一个大质粒（ti质粒，肿瘤诱导质粒）上，参与了DNA转移到宿主细胞基因组的不同步骤：细菌毒力的激活，T-DNA及其相关蛋白的合成和输出，宿主细胞内T-DNA和效应蛋白的细胞内运输，以及T-DNA在宿主基因组DNA中的整合。在感染过程中，这些细菌编码蛋白与宿主因子之间发生多种相互作用，这决定了感染的结果。在这里，我们回顾了目前关于细菌和植物因素控制农杆菌毒力和寄主植物易感性的机制的知识。
Several species of the Agrobacterium genus represent unique bacterial pathogens able to genetically transform plants, by transferring and integrating a segment of their own DNA (T-DNA, transferred DNA) in their host genome. Whereas in nature this process results in uncontrolled growth of the infected plant cells (tumors), this capability of Agrobacterium has been widely used as a crucial tool to generate transgenic plants, for research and biotechnology. The virulence of Agrobacterium relies on a series of virulence genes, mostly encoded on a large plasmid (Ti-plasmid, tumor inducing plasmid), involved in the different steps of the DNA transfer to the host cell genome: activation of bacterial virulence, synthesis and export of the T-DNA and its associated proteins, intracellular trafficking of the T-DNA and effector proteins in the host cell, and integration of the T-DNA in the host genomic DNA. Multiple interactions between these bacterial encoded proteins and host factors occur during the infection process, which determine the outcome of the infection. Here, we review our current knowledge of the mechanisms by which bacterial and plant factors control Agrobacterium virulence and host plant susceptibility.