Decreased Proliferation of Aging Tenocytes Is Associated with Down-Regulation of Cellular Senescence-Inhibited Gene and Up-Regulation of p27
Decreased Proliferation of Aging Tenocytes Is Associated with Down-Regulation of Cellular Senescence-Inhibited Gene and Up-Regulation of p27
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DOI：
10.1002/jor.21418
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发表时间：
2011-10-01
影响因子：
2.8
通讯作者：
Pang, Jong-Hwei S.
中科院分区：
文献类型：
--
作者：
Tsai, Wen-Chung;Chang, Hsiang-Ning;Pang, Jong-Hwei S.
症状性肌腱病往往与年龄有关。然而，衰老的分子机制及其对肌腱细胞增殖和细胞周期进程的影响尚不清楚。我们检查了三个年龄组（年轻，2个月;中年，12个月，和近衰老，24个月）的大鼠跟腱的腱细胞。通过3-[4，5-二甲基噻唑-2-基]-2，5-二苯基溴化四氮唑（MTT）测定和流式细胞术评估腱细胞增殖。衰老相关的β-半乳糖苷酶（SA β-gal）染色进行所有组的肌腱细胞。逆转录-聚合酶链反应（RT-PCR）检测细胞衰老抑制基因（CSIG）mRNA表达，Western blot检测p27蛋白表达。MTT法检测结果显示，肌腱细胞增殖能力随年龄增长而下降（p < 0.05）。衰老腱细胞的细胞周期进程被阻滞在G 0/G1期。更多的衰老肌腱细胞表达SA β-gal比年轻的肌腱细胞。通过RT-PCR和Western blot分析发现，随着年龄的增长，CSIG基因和蛋白表达下调，而p27基因和蛋白表达上调。总之，肌腱细胞的增殖随着年龄的增长而下降，并与CSIG的下调和p27的上调有关。(C)2011年由Wiley Periodicals，Inc.出版的骨科研究学会。J Orthop Res 29：1598-1603，2011
Symptomatic tendinopathy tends to be age-related. However, the molecular mechanisms of ageing and its effects on tenocyte proliferation and cell cycle progression are unknown. We examined tenocytes from Achilles tendons in rats from three age groups (young, 2 months; middle-aged, 12 months, and near senescence, 24 months). Tenocyte proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. Senescence-associated beta-galactosidase (SA beta-gal) staining was performed in all groups of tenocytes. mRNA and protein expression of cellular senescence-inhibited gene (CSIG) and p27 was measured by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The results of MTT assay revealed that tenocyte proliferation decreased with age (p < 0.05). Cell cycle progression was arrested at G0/G1 phase in senescent tenocytes. More senescent tenocytes expressed SA beta-gal than young tenocytes did. By RT-PCR and Western blot analysis, the gene and protein expression of CSIG was found to be down-regulated, whereas that of p27 was up-regulated with age. In conclusion, the proliferation of tenocytes declines with age and is associated with the down-regulation of CSIG and up-regulation of p27. (C) 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:1598-1603, 2011