DRAMATIC GROWTH OF MICE THAT DEVELOP FROM EGGS MICRO-INJECTED WITH METALLOTHIONEINE-GROWTH HORMONE FUSION GENES
DRAMATIC GROWTH OF MICE THAT DEVELOP FROM EGGS MICRO-INJECTED WITH METALLOTHIONEINE-GROWTH HORMONE FUSION GENES
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DOI：
10.1038/300611a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
EVANS, RM
中科院分区：
文献类型：
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作者：
PALMITER, RD;BRINSTER, RL;EVANS, RM
将含有小鼠金属硫蛋白-I基因启动子与大鼠生长激素结构基因融合的DNA片段显微注射到受精小鼠卵的原核中。在从这些卵发育而来的21只小鼠中，有7只携带融合基因，其中6只明显比同窝出生的小鼠大。这些转基因小鼠中有几只在肝脏中有非常高水平的融合mRNA，在血清中有非常高水平的生长激素。这种方法对研究生长激素的生物学效应有影响，作为一种加速动物生长的方法，作为一种模型，作为一种纠正遗传疾病的手段，作为一种种植有价值的基因产品的方法。
A DNA fragment containing the promoter of the mouse metallothionein-I gene fused to the structural gene of rat growth hormone was microinjected into the pronuclei of fertilized mouse eggs. Of 21 mice that developed from these eggs, 7 carried the fusion gene and 6 of these grew significantly larger than their littermates. Several of these transgenic mice had extraordinarily high levels of the fusion mRNA in their liver and growth hormone in their serum. This approach has implications for studying the biological effects of growth hormone, as a way to accelerate animal growth, as a model for gigantism, as a means of correcting genetic disease and as a method of farming valuable gene products.