Enzymatic Enantioselective Hydrolysis of 2‑(3-Chlorophenyl) Propionic Acid Ester Enhanced by PEG: Experiment and Optimization
Enzymatic Enantioselective Hydrolysis of 2‑(3-Chlorophenyl) Propionic Acid Ester Enhanced by PEG: Experiment and Optimization
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PEG 增强的 2-(3-氯苯基) 丙酸酯酶促对映选择性水解：实验与优化
DOI：
10.1021/acs.iecr.8b02377
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发表时间：
2018
影响因子：
4.2
通讯作者：
唐课文
中科院分区：
文献类型：
--
作者：
张盼良;成晴;许卫风;唐课文
报道了洋葱假单胞菌脂肪酶PS催化2-（3-氯苯基）丙酸酯水解拆分2-（3-氯苯基）丙酸（CPA）的方法。以聚乙二醇（PEG 400）为助溶剂，底物转化率提高了88%，具有较高的对映选择性。考察了脂肪酶种类、底物结构、共溶剂、温度、pH、酶用量、PEG 400浓度、底物浓度、反应时间等因素对酶活力的影响，得到最佳反应条件为：15 mg mL-1脂肪酶PS，20 mmol L-1底物，30%（m/m）PEG 400，在70 °C、pH 6.5条件下反应36 h，对映体过量和总转化率分别可达99.02%和42.09%。采用非竞争性抑制动力学模型对反应过程进行了模拟，模型预测值与实验数据吻合较好，这对深入理解反应机理，进而指导工艺设计和操作具有重要意义。
Chiral resolution of 2-(3-chlorophenyl) propionic acid (CPA) via the hydrolysis of CPA ester catalyzed by lipase PS fromPseudomonas cepaciais reported. With polyethylene glycol (PEG 400) as a cosolvent, the substrate conversion is increased by 88% with high enantioselectivity. Important factors including lipase type, substrate structure, cosolvent, temperature, pH, enzyme loading, PEG 400 and substrate concentrations, and reaction time were investigated, and the optimal conditions including 15 mg mL–1lipase PS, 20 mmol L–1substrate, 30% (m/m) PEG 400, reaction for 36 h at 70 °C and pH 6.5 were obtained, where the enantiomeric excess and overall conversion rate could reach up to 99.02% and 42.09%, respectively. An uncompetitive inhibition kinetic model was applied to simulate the reaction process, and the model predictions are in good agreement with the experimental data, which is important for a deep understanding of the reaction mechanism, and then for process design and operation.