Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis.
Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis.
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空肠弯曲菌DsbA1、DsbA2与C8J_1298周质氧化还原酶的相互作用及其对细菌生理和致病的影响
DOI：
10.3390/ijms222413451
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发表时间：
2021-12-15
影响因子：
5.6
通讯作者：
Jagusztyn-Krynicka EK
中科院分区：
文献类型：
--
作者：
Banaś AM;Bocian-Ostrzycka KM;Dunin-Horkawicz S;Ludwiczak J;Wilk P;Orlikowska M;Wyszyńska A;Dąbrowska M;Plichta M;Spodzieja M;Polańska MA;Malinowska A;Jagusztyn-Krynicka EK
Dsb家族的细菌蛋白催化半胱氨酸残基之间二硫键的形成，从而稳定蛋白质结构并确保其正常功能。在这里，我们报告的空肠弯曲菌的Dsb途径的详细分析。该病原体的氧化Dsb系统是独特的，因为它由两个单体DsbA（DsbA1和DsbA2）和一个二聚体双功能蛋白（C8J_1298）组成。之前，我们证明了DsbA1和C8J_1298是冗余的。在这里，我们解开了两个单体DsbAs之间的相互作用，通过在体外和体内实验，并通过解决他们的结构，发现这两个单体DsbAs是cytoplasmic蛋白。它们的结构证实它们是EcDsbL的同源物。在蛋白质的表面电荷中观察到的轻微差异不影响与其氧化还原配偶体的相互作用。比较蛋白质组学表明，几个呼吸蛋白，以及周质转运蛋白，DSB系统的目标。其中一些，包括电子供体和电子受体，是C的基本元素。空肠呼吸过程在宿主肠道中的氧限制条件下。所提供的数据提供了关于C的功能的详细信息。空肠Dsb系统，确定其为新型抗菌分子的潜在靶标。
The bacterial proteins of the Dsb family catalyze the formation of disulfide bridges between cysteine residues that stabilize protein structures and ensure their proper functioning. Here, we report the detailed analysis of the Dsb pathway of Campylobacter jejuni. The oxidizing Dsb system of this pathogen is unique because it consists of two monomeric DsbAs (DsbA1 and DsbA2) and one dimeric bifunctional protein (C8J_1298). Previously, we showed that DsbA1 and C8J_1298 are redundant. Here, we unraveled the interaction between the two monomeric DsbAs by in vitro and in vivo experiments and by solving their structures and found that both monomeric DsbAs are dispensable proteins. Their structures confirmed that they are homologs of EcDsbL. The slight differences seen in the surface charge of the proteins do not affect the interaction with their redox partner. Comparative proteomics showed that several respiratory proteins, as well as periplasmic transport proteins, are targets of the Dsb system. Some of these, both donors and electron acceptors, are essential elements of the C. jejuni respiratory process under oxygen-limiting conditions in the host intestine. The data presented provide detailed information on the function of the C. jejuni Dsb system, identifying it as a potential target for novel antibacterial molecules.
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DOI：
10.1099/13500872-145-8-2145
发表时间：
1999-08-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Bleumink-Pluym, NMC;Verschoor, F;Fry, BN
通讯作者：
Fry, BN
DOI：
10.1073/pnas.90.3.1043
发表时间：
1993-02-01
影响因子：
11.1
作者：
DAILEY, FE;BERG, HC
通讯作者：
BERG, HC
影响因子：
5.2
作者：
Bocian-Ostrzycka KM;Łasica AM;Dunin-Horkawicz S;Grzeszczuk MJ;Drabik K;Dobosz AM;Godlewska R;Nowak E;Collet JF;Jagusztyn-Krynicka EK
通讯作者：
Jagusztyn-Krynicka EK
影响因子：
7.3
作者：
Duprez, Wilko;Premkumar, Lakshmanane;Martin, Jennifer L.
通讯作者：
Martin, Jennifer L.
DOI：
10.1073/pnas.1518952113
发表时间：
2016-03-29
影响因子：
11.1
作者：
Beeby, Morgan;Ribardo, Deborah A.;Hendrixson, David R.
通讯作者：
Hendrixson, David R.