Effects of n-propyl gallate on neuronal survival after forebrain ischemia in rats
Effects of n-propyl gallate on neuronal survival after forebrain ischemia in rats
复制标题
DOI：
10.1016/j.resuscitation.2011.07.024
复制
发表时间：
2012-02-01
期刊：
影响因子：
6.5
通讯作者：
Furuya, Hitoshi
中科院分区：
文献类型：
--
作者：
Kawano, Yasunobu;Kawaguchi, Masahiko;Furuya, Hitoshi
目的：研究没食子酸丙酯(PG)对大鼠前脑缺血后海马神经元存活的影响。方法：40只雄性SD大鼠随机分为6组。PG-I-10、PG-I-8、PG-S组分别在重度(10min)、中度(8min)缺血或假手术前72、48、24、30min腹腔注射PG(100 mg/kg)，V-I-10、V-I-8、V-S组给予10%二甲基亚砜。异氟醚麻醉下双侧颈总动脉结扎联合降压(35 Mm Hg)造成大鼠前脑缺血。再灌流7d后处死动物。苏木精-伊红染色进行组织学评价。结果：PG-I-8组正常神经元数量明显高于V-I-8组，而PG-I-10组与V-I-10组无明显差异。接受PG的动物HIF-1α、EPO和VEGF的表达水平显著高于接受赋形剂的动物。结论：在中度但不严重的前脑缺血模型中，给予PG可能具有神经保护作用。(C)2011爱思唯尔爱尔兰有限公司。保留所有权利。
The aim of the study: The present study was conducted to assess the effects of intraperitoneal administration of n-propyl gallate (PG) on hippocampal neuronal survival after forebrain ischemia.Methods: Forty male Sprague-Dawley rats were randomly assigned to one of 6 groups. Animals in the PG-I- 10, PG-I-8 and PG-S groups received intraperitoneal injection of PG (100 mg/kg) 72, 48, 24 h and 30 min before severe (10 min) or moderate (8 min) ischemia or sham operation, respectively, while animals in the V-I-10, V-I-8 and V-S groups received the vehicle (10% DMSO) in the same manner. Forebrain ischemia was produced by bilateral carotid occlusion combined with hypotension (35 mmHg) under isoflurane anesthesia. Animals were killed 7 days after reperfusion. Histological assessments were performed using hematoxylin and eosin staining. In separate groups of animals that received PG or vehicle, m-RNA levels of hypoxia-inducible factor 1 alpha (HIF-1 alpha), erythropoietin (EPO) and vascular endothelial growth factor (VEGF) were measured using the reverse transcription-PCR protocol.Results: The number of normal neurons was significantly higher in the PG-I-8 group compared with that in the V-I-8 group, whereas it was similar between the PG-I-10 and V-I-10 groups. Animals that received PG had significantly higher levels of HIF-1 alpha, EPO and VEGF expression compared with those that received vehicle.Conclusion: The results indicated that intraperitoneal administration of PG may have neuroprotective effects in a model of moderate, but not severe, forebrain ischemia in rats. (C) 2011 Elsevier Ireland Ltd. All rights reserved.