Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces.
Analysis of bovine serum albumin adsorption on calcium phosphate and titanium surfaces.
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DOI：
10.1016/s0142-9612(98)00184-7
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发表时间：
1999-02
期刊：
影响因子：
14
通讯作者：
H. Zeng;K. Chittur;W. Lacefield
中科院分区：
文献类型：
--
作者：
H. Zeng;K. Chittur;W. Lacefield
研究了磷酸钙（CaP）生物陶瓷与钛（Ti）复合薄膜对蛋白质的吸附行为。采用离子束溅射沉积技术，以羟基磷灰石（HA）、氟磷灰石（FA）和钛（Ti）为靶制备薄膜。傅里叶变换红外光谱（FTIR）与衰减全内反射（ATR）被用来评估蛋白质吸附在这些表面上。研究表明，表面组成和结构影响蛋白质吸附动力学和吸附蛋白质的结构。CaP表面比Ti表面吸附更多的蛋白质，并引起更多的改变吸附BSA的结构比Ti表面。蛋白质吸附行为的差异可能导致CaP和Ti植入物表面上非常不同的初始细胞行为。
The protein adsorption behavior of thin films of calcium phosphate (CaP) bioceramic and titanium (Ti) was studied in this research. The thin films were produced with an ion beam sputter deposition technique using targets of hydroxyapatite (HA), fluorapatite (FA) and titanium (Ti). Fourier transform infrared spectroscopy (FTIR) with attenuated total internal reflectance (ATR) was used to evaluate protein adsorption on these surfaces. This study showed that surface composition and structure influenced the kinetics of protein adsorption and the structure of adsorbed protein. CaP surfaces adsorbed greater amount of protein than the Ti surface, and caused more alteration of the structure of adsorbed BSA than did the Ti surface. The differences in protein adsorption behavior could result in very different initial cellular behavior on CaP and Ti implant surfaces.