Aflatoxin B1 degradation by Bacillus subtilis UTBSP1 isolated from pistachio nuts of Iran
Aflatoxin B1 degradation by Bacillus subtilis UTBSP1 isolated from pistachio nuts of Iran
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DOI：
10.1016/j.foodcont.2011.06.018
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发表时间：
2012-01-01
期刊：
影响因子：
6
通讯作者：
Javan-Nikkhah, Mohammad
中科院分区：
文献类型：
--
作者：
Farzaneh, Mohsen;Shi, Zhi-Qi;Javan-Nikkhah, Mohammad
开心果是伊朗黄曲霉毒素污染风险最高的商品之一。黄曲霉毒素B1（AFB 1）是对人类和家畜危害最大的真菌毒素之一。在自然界中，存在能够减少食品和饲料产品中的黄曲霉毒素污染的微生物。本研究从阿月浑子中分离得到一株枯草芽孢杆菌UTBSP 1，并对其降解黄曲霉毒素B1进行了研究。采用高效薄层色谱法（HPTLC）、高效液相色谱法（HPLC）和LC-MS/MS多反应监测法（MRM）测定黄曲霉毒素B1的含量，结果为B。subtilis uTBSP 1对黄曲霉毒素B1的去除率为85.66%，对阿月浑子的去除率为0.5%。无细胞上清液使AFB_1含量下降78.39%。无菌上清降解黄曲霉毒素B_1的最适条件为35-40 ℃，降解时间为24 h。此外，结果表明，黄曲霉毒素B1的降解是酶和负责酶的细胞外和组成型产生。破坏性AFB 1与标准AFB 1在化学上不同，并且失去了荧光性质。(C)2011爱思唯尔有限公司保留所有权利。
Pistachio nuts are among the commodities with the highest risk of aflatoxin contamination in Iran. Aflatoxin B1 (AFB1) is one of the most hazardous mycotoxins for humans and livestock. In nature, there are microorganisms which are capable of reducing aftatoxins contamination in food and feed products. In this study, Bacillus subtilis strain UTBSP1 was isolated from pistachio nuts and studied for the degradation of AFB1. The AFB1 contents were determined by the use of HPTLC and HPLC as well as multiple reactions monitoring (MRM) method in LC-MS/MS. The results indicated B. subtilis uTBSP1 could considerably remediate AFB1 from nutrient broth culture and pistachio nut by 85.66% and 05%, respectively. Cell free supernatant fluid caused an apparent 78.39% decrease in AFB1 content. The optimal conditions for AFB1 degradation by cell free supernatant appeared at 35-40 degrees C, during 24 h. Furthermore, the results indicated that AFB1 degradation is enzymatic and responsible enzymes are extracellular and constitutively produced. The destructive AFB1 differed from standard AFB1 chemically, and lost a fluorescence property. (C) 2011 Elsevier Ltd. All rights reserved.