Protein-resistant polymer coatings on silicon oxide by surface-initiated atom transfer radical polymerization
Protein-resistant polymer coatings on silicon oxide by surface-initiated atom transfer radical polymerization
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DOI：
10.1021/la052796r
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发表时间：
2006-04-11
期刊：
影响因子：
3.9
通讯作者：
Chilkoti, A
中科院分区：
文献类型：
--
作者：
Ma, HW;Li, DJ;Chilkoti, A
用聚乙二醇对氧化硅进行改性以有效消除蛋白质吸附已被证明在技术上具有挑战性。在本文中，我们证明了表面引发的原子转移自由基聚合（SI-ATRP）的寡（乙二醇）甲基丙烯酸甲酯（OEGMA）成功地产生聚合物涂层的氧化硅，具有优异的蛋白质抗性在生物环境中。这些涂层的血清吸附油的水平低于椭圆偏振法的检测限。我们还展示了一种新的软光刻方法，通过该方法将SI-ATRP与微接触印刷相结合，以创建聚（OEGMA）的微图案，该微图案可以在空间上引导蛋白质在基底的裸露区域上的吸附。这种方法的合奏将是有用的，在筛选生物相互作用，其中非特异性结合必须被抑制，以辨别低概率结合事件从复杂的混合物和图案的锚定依赖性细胞的玻璃和氧化硅。
The modification of silicon oxide with poly(ethylene glycol) to effectively eliminate protein adsorption has proven to be technically challenging. In this paper, we demonstrate that surface-initiated atom transfer radical polymerization (SI-ATRP) of oligo(ethylene glycol) methyl methacrylate (OEGMA) successfully produces polymer coatings on silicon oxide that have excellent protein resistance in a biological milieu. The level of serum adsorption oil these coatings is below the detection limit of ellipsometry. We also demonstrate a new soft lithography method via which SI-ATRP is integrated with microcontact printing to create micropatterns of poly(OEGMA) on class that can spatially direct the adsorption of proteins on the bare regions of the substrate. This ensemble of methods will be useful in screening biological interactions where nonspecific binding must be suppressed to discern low probability binding events from a complex mixture and to pattern anchorage-dependent cells on glass and silicon oxide.