TRANSPORT EPITHELIAL CHARACTERISTICS OF CULTURED BOVINE PITUITARY FOLLICULAR CELLS
TRANSPORT EPITHELIAL CHARACTERISTICS OF CULTURED BOVINE PITUITARY FOLLICULAR CELLS
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DOI：
10.1152/ajpendo.1987.252.3.e304
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发表时间：
1987-03-01
影响因子：
--
通讯作者：
WEINER, RI
中科院分区：
文献类型：
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作者：
FERRARA, N;FUJII, DK;WEINER, RI
从酶分散和机械分散的牛前脑垂体部(AP)和结节部(PT)获得融合的单层多边形上皮样细胞。单层细胞的超微结构与垂体的滤泡或滤泡星状细胞(FC)一致，即缺乏分泌颗粒；在培养过程中形成滤泡；与相邻细胞交错排列，有无数紧密连接；存在广泛的微丝；从第一代培养的单层培养上清液中几乎没有AP激素、催乳素和ACTH。在达到汇合后不久，单层的区域从培养皿的表面凸出，形成圆顶。只有在体内具有运输上皮功能的细胞培养才能描述穹顶的形成。将培养在聚碳酸酯滤膜上的FC置于Ussing小室中。跨上皮电势差为.apprx。在电镀后4-5天，可检测到1.1 mV和大于300Ω·cm2的电阻。应用于粘膜表面的阿米洛利可使短路电流(ISC)降低70%，而在浆膜表面加入哇巴因可进一步降低ISC。β-肾上腺素能激动剂异丙肾上腺素增加ISC，这一作用可被β-受体拮抗剂阻断。这些观察结果表明，培养的垂体Fc具有运输上皮的作用。考虑到Fc在AP和PT中的组织结构，他们建议Fc在维持脑下垂体间质液的离子组成方面发挥调节作用。
Confluent monolayers of polygonal epithelioid cells were obtained from enzymatically and mechanically dispersed bovine anterior pituitaries (AP) and pars tuberali (PT). The ultrastructure of the cells composing the monolayer was consistent with the follicular or folliculostellate cells (FC) of the pituitary, i.,e., lack of secretory granules; formation of follicles in culture; interdigitations with neighoring cells with numeruous tight junctions; presence of extensive microfilaments; and sparse rough endoplasmic reticulum and Golgi appratus. Culture media from monolayers of first passage cultures contained little if any of the AP hormones'' luteinizing hormone, prolactin, and ACTH. Shortly after reaching confluency, regions of the monolayer bulge away from the surface of the culture dish to form domes. Dome formation has been described only with cultures of cells that function as transport epithelia in vivo. FC cultured on polycarbonate filter were placed in Ussing chambers. A transepithelial potential difference of .apprx. 1.1 mV and a resistance greater than 300 .OMEGA.cm2 were detectable 4-5 days after plating. The short-circuit current (Isc) was decreased 70% by amiloride applied to the mucosal surface and further decreased by the addition of ouabain at the serosal surface. The .beta.-adrenergic agonist isoproterenol increased the Isc and this action was prevented by a .beta.-antagonist. These observations indicate that pituitary FC in culture behave as a transport epithelium. Considering the organization of FC in the AP and PT, they suggest a regulatory role for FC in the maintenance of the ionic composition of the interstitial fluid of the pituitary gland.