Field collection of Nasonia (parasitoid wasp) using baits.
Field collection of Nasonia (parasitoid wasp) using baits.
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使用诱饵现场采集 Nasonia（寄生蜂）。
DOI：
10.1101/pdb.prot5313
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发表时间：
2009
影响因子：
--
通讯作者：
Loehlin,DavidW
中科院分区：
文献类型：
--
作者：
Werren,JohnH;Loehlin,DavidW
该方案描述了一种使用“蝇肝”收集纳苏尼亚黄蜂的标准方法：肝脏残留物由蝇蛆（例如，肉蝇属）喂养，并在成熟幼虫分散后收集。蝇肝含有一种对黄蜂非常有吸引力的挥发性物质，将其放置在一个大（约15平方厘米）的网袋中，挂在适当的收集点（例如，鸟巢附近或涵洞下）。在大网袋内是一个较小的网袋，放置在紧靠蝇肝处，装有4至6个肉蝇属。这些袋子会留住黄蜂，黄蜂会进入袋子并开始叮咬宿主。网眼袋可以用标准的尼龙窗纱或任何其他材料制成，网眼宽度足以允许黄蜂进入。
This protocol describes a standard method for collecting Nasonia wasps using “flyliver”: liver remains fed upon by fly maggots (eg, Sarcophaga) and collected after the mature larvae have dispersed. The flyliver, which contains a volatile substance that is very attractive to the wasps, is placed in a large (~ 15-cm2) mesh bag hung in appropriate collection spots (eg, near birds’ nests or under culverts). Within the large mesh bag is a smaller mesh bag placed abutting the flyliver and containing four to six Sarcophaga pupae. These retain the wasps, which will enter the bag and begin stinging the hosts. The mesh bags can be made with standard nylon window screening, or any other material with mesh width large enough to permit entry of the wasps.