Bacterin That Induces Anti-OspA and Anti-OspC Borreliacidal Antibodies Provides a High Level of Protection against Canine Lyme Disease
Bacterin That Induces Anti-OspA and Anti-OspC Borreliacidal Antibodies Provides a High Level of Protection against Canine Lyme Disease
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DOI：
10.1128/cvi.00373-08
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发表时间：
2009-02-01
影响因子：
--
通讯作者：
Schell, Ronald F.
中科院分区：
文献类型：
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作者：
LaFleur, Rhonda L.;Dant, Jennifer C.;Schell, Ronald F.
每组15只实验室饲养的比格犬接种疫苗，并用安慰剂或佐剂化的二价双链螺旋体蛋白（由传统的伯氏疏螺旋体菌株和独特的ospA和ospB阴性B组成）加强免疫。表达高水平OspC，然后用B攻击的burgdorferi菌株。感染了伯氏硬蜱的肩突硬蜱接种疫苗的狗产生高滴度的抗OspA和抗OspC杀疏螺旋体抗体，包括对在致病性疏螺旋体基因种中保守的OspC羧基末端（称为OspC7）的最后七个氨基酸内的表位具有特异性的杀疏螺旋体抗体。此外，从感染的蜱虫中消除了螺旋体，这些蜱虫进食了受体B。没有从皮肤或关节中分离出伯氏菌，抗体反应与犬感染B特异性相关。没有生产burgdorferi。相反，B.从13只（87%）安慰剂疫苗接种犬（P <0.0001）的饱食蜱、14只（93%）犬的皮肤活检标本（P <0.0001）和8只（53%）犬的关节组织标本（P = 0.0022）中回收到伯氏螺旋体。此外，14只（93%）犬产生了针对B的特异性抗体应答。11例（73%）为C6肽抗体阳性（P <0.0001）。此外，10只（67%）犬出现莱姆病相关关节异常（P <0.0001），包括4只（27%）犬出现关节僵硬或跛行，6只（40%）犬出现慢性关节炎症（滑膜炎）。因此，结果证实，在免疫后不久，抗莱姆病蛋白提供了高水平的保护。
Groups of 15 laboratory-bred beagles were vaccinated and boosted with either a placebo or adjuvanted bivalent bacterin comprised of a traditional Borrelia burgdorferi strain and a unique ospA- and ospB-negative B. burgdorferi strain that expressed high levels of OspC and then challenged with B. burgdorferi-infected Ixodes scapularis ticks. The vaccinated dogs produced high titers of anti-OspA and anti-OspC borreliacidal antibodies, including borreliacidal antibodies specific for an epitope within the last seven amino acids at the OspC carboxy terminus (termed OspC7) that was conserved among pathogenic Borrelia genospecies. In addition, spirochetes were eliminated from the infected ticks that fed on the bacterin recipients, B. burgdorferi was not isolated from the skin or joints, and antibody responses associated specifically with canine infection with B. burgdorferi were not produced. In contrast, B. burgdorferi was recovered from engorged ticks that fed on 13 (87%) placebovaccinated dogs (P < 0.0001), skin biopsy specimens from 14 (93%) dogs (P < 0.0001), and joint tissue specimens from 8 (53%) dogs (P = 0.0022). In addition, 14 (93%) dogs developed specific antibody responses against B. burgdorferi proteins, including 11 (73%) with C6 peptide antibodies (P < 0.0001). Moreover, 10 (67%) dogs developed Lyme disease-associated joint abnormalities (P < 0.0001), including 4 (27%) dogs that developed joint stiffness or lameness and 6 (40%) that developed chronic joint inflammation (synovitis). The results therefore confirmed that the bacterin provided a high level of protection against Lyme disease shortly after immunization.