First successful vitrification of salmonid ovarian tissue
First successful vitrification of salmonid ovarian tissue
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DOI：
10.1016/j.cryobiol.2017.04.005
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发表时间：
2017-06-01
期刊：
影响因子：
2.7
通讯作者：
Horvath, Akos
中科院分区：
文献类型：
--
作者：
Lujic, Jelena;Marinovic, Zoran;Horvath, Akos
由于缺乏鱼卵和胚胎的冷冻保存协议，替代技术，这将使女性遗传资源的存储是至关重要的保护生物学和水产养殖业的生殖管理的未来发展。本试验旨在建立一种适合于褐鳟萨尔莫幼鱼卵巢组织超低温保存的玻璃化冷冻方案。使用针浸玻璃化（NIV）方法，其中将卵巢固定在针灸针上，通过含有不同组合和浓度的甲醇（MeOH）、丙二醇（PG）和二甲基亚砜（Me2SO）的平衡和玻璃化溶液，随后浸入液氮中。玻璃化溶液中含有相等的冷冻保护剂浓度（3M Me2SO和3M PG）产生最高的卵原细胞存活率（高达40%）和定性和定量不变的perinucleolar卵泡。为褐鳟鱼开发的方法可应用于其他鲑科鱼类的雌性遗传资源的保护，包括濒危和特有物种或种群。(C)2017爱思唯尔公司All rights reserved.
Due to a lack of cryopreservation protocols for fish eggs and embryos, alternative techniques which will enable storage of female genetic resources are crucial for future development of reproduction management in conservation biology and aquaculture. Experiments were conducted to develop an optimal vitrification protocol for cryopreservation of brown trout Salmo trutta juvenile ovarian tissue. Needle immersed vitrification (NIV) method was used where ovaries were pinned on an acupuncture needle, passaged through equilibration and vitrification solutions containing different combinations and concentrations of methanol (MeOH), propylene glycol (PG) and dimethyl sulfoxide (Me2SO) and subsequently plunged into liquid nitrogen. Vitrification solutions containing equal cryoprotectant concentrations (3M Me2SO and 3M PG) yielded the highest oogonia survival rates (up to 40%) and qualitatively and quantitatively unaltered perinucleolar follicles. The method developed for brown trout could be applied to the conservation of female genetic resources of other salmonid species, including endangered and endemic species or populations. (C) 2017 Elsevier Inc. All rights reserved.