Detection and quantification of soy allergens in food: Study of two commercial enzyme-linked immunosorbent assays
Detection and quantification of soy allergens in food: Study of two commercial enzyme-linked immunosorbent assays
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DOI：
10.1111/j.1750-3841.2007.00298.x
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发表时间：
2007-04-01
影响因子：
3.9
通讯作者：
Munyana, Christelia
中科院分区：
文献类型：
--
作者：
L'Hocine, Lamia;Boye, Joyce Irene;Munyana, Christelia
本研究的目的是评估两种市售大豆酶联免疫吸附试验（ELISA）的有效性，并使用它们检测选定的食品中的大豆蛋白。两种ELISA试剂盒均具有较高的灵敏度。测定的检测限（LOD）（约为2%和47%）。非变性和非加热的大豆分离蛋白（SPI）样品的抗原性也显着低于处理过的。
The objective of this study was to evaluate the efficacy of 2 commercially available soy enzyme-linked immunosorbent assays (ELISA) and use them in detecting soy proteins in selected food commodities. Both ELISA kits exhibited high sensitivity. The determined limits of detection (LOD) (approximately 2 and 47%). Nondenatured and nonheated soy protein isolate (SPI) samples were also significantly less antigenic than the treated ones.