ADSORPTION OF FIBRONECTIN AND VITRONECTIN ONTO PRIMARIA(TM) AND TISSUE-CULTURE POLYSTYRENE AND RELATIONSHIP TO THE MECHANISM OF INITIAL ATTACHMENT OF HUMAN VEIN ENDOTHELIAL-CELLS AND BHK-21 FIBROBLASTS
ADSORPTION OF FIBRONECTIN AND VITRONECTIN ONTO PRIMARIA(TM) AND TISSUE-CULTURE POLYSTYRENE AND RELATIONSHIP TO THE MECHANISM OF INITIAL ATTACHMENT OF HUMAN VEIN ENDOTHELIAL-CELLS AND BHK-21 FIBROBLASTS
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DOI：
10.1016/0142-9612(95)98901-p
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发表时间：
1995-09-01
期刊：
影响因子：
14
通讯作者：
UNDERWOOD, PA
中科院分区：
文献类型：
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作者：
STEELE, JG;DALTON, BA;UNDERWOOD, PA
比较了两种细胞培养基质，组织培养聚苯乙烯（TCPS）和PrimariaTM，以确定含氮表面（如Primaria）是否通过与含氧表面（TCPS）不同的机制吸引细胞。测定了Primaria和TCPS从培养基中胎牛血清（FBS）组分中吸附的玻连蛋白（Vn）和纤连蛋白（Fn）的量，Primaria吸附的Fn是TCPS的2 ~ 3倍，但从含FBS的培养基中吸附的Vn量相似。在Primaria上的Fn和Vn结合位点是不同的，因为这两种蛋白质之间的吸附是非竞争性的。吸附到两个表面上的Fn和Vn的量与Fn和Vn的细胞附着活性的浓度依赖性进行比较。尽管吸附到TCPS上的Fn的量对于细胞附着是次优的，但Primaria吸附的Fn表面密度对于人静脉内皮细胞和BHK-21成纤维细胞的附着是超优的。我们得出结论，Primaria TCPS不同，Fn和Vn介导初始细胞附着Primaria时，培养基中含有FBS，而细胞附着TCPS是依赖于Vn。
The two cell culture substrata, tissue culture polystyrene (TCPS) and PrimariaTM were compared in order to determine whether a nitrogen-containing surface such as Primaria attracts cells by a different mechanism to an oxygen-containing surface (TCPS). The amounts of vitronectin (Vn) and fibronectin (Fn) which adsorb from the fetal bovine serum (FBS) component of the culture medium onto Primaria and TCPS were determined, Primaria adsorbed two- to threefold more Fn than TCPS, but adsorbed similar amounts of Vn from medium containing FBS. The Fn and Vn binding sites on Primaria were distinct, as adsorption was non-competitive between these two proteins. The amounts of Fn and Vn that adsorbed onto the two surfaces were compared to the concentration dependence of the cell attachment activities of Fn and Vn. Whereas the amounts of Fn which adsorbed onto TCPS were suboptimal for cell attachment, Primaria adsorbed an Fn surface density that was supraoptimal for attachment of human vein endothelial cells and BHK-21 fibroblasts. We conclude that Primaria differs from TCPS in that both Fn and Vn mediate initial cell attachment to Primaria when the culture medium contains FBS, whereas cell attachment to TCPS is dependent upon Vn.