VECTORIAL ORIENTATION OF HUMAN MONO-AMINE OXIDASE IN THE MITOCHONDRIAL OUTER-MEMBRANE
VECTORIAL ORIENTATION OF HUMAN MONO-AMINE OXIDASE IN THE MITOCHONDRIAL OUTER-MEMBRANE
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DOI：
10.1042/bj1810007
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发表时间：
1979-01-01
影响因子：
4.1
通讯作者：
MAYER, RJ
中科院分区：
文献类型：
--
作者：
RUSSELL, SM;DAVEY, J;MAYER, RJ
研究了单胺氧化酶（EC 1.4.3.4）在人肝脏线粒体和脑皮质非突触体和突触体线粒体制备过程中线粒体外膜的定位。利用等渗透和低渗透线粒体制剂的免疫化学可及性来定位酶。免疫化学可及的酪胺氧化活性在人肝脏和脑皮质非突触体线粒体膜的两个表面上分布近似均匀。免疫化学上可接近的。在肝脏和脑皮质非突触体线粒体制剂中，-苯乙胺氧化活性主要位于外表面，免疫化学可及的5-羟色胺氧化活性主要位于线粒体外膜的内表面。在突触体线粒体的制备过程中，酶的分布有相当大的变化。与观察结果相一致的最简单的模型是，在肝脏和大脑皮层的非突触体线粒体中，酪胺氧化活性分布在线粒体外膜的两侧。-苯乙胺氧化活性位于外膜的外表面，5-羟色胺氧化活性位于线粒体外膜的内表面。
The localization of monoamine oxidase (EC 1.4.3.4) in the mitochondrial outer membrane was studied in preparations of human liver mitochondrial and brain-cortex non-synaptosomal and synaptosomal mitochondria. Immunochemical accessibility in iso-osmotic and hypo-osmotic mitochondrial preparations was used to localize the enzyme. The immunochemically accessible tyramine-oxidizing activity was distributed approximately equally on both surfaces of the membrane in human liver and brain-cortex non-synaptosomal mitochondria. The immunochemically accessible .beta.-phenethylamine-oxidizing activity was situated predominantly on the outer surface and the immunochemically accessible 5-hydroxytryptamine-oxidizing activity was situated predominantly on the inner surface of the mitochondrial outer membrane in liver and brain-cortex non-synaptosomal mitochondrial preparations. Considerable variation in the distribution of the enzyme in preparations of synaptosomal mitochondria was seen. The simplest model consistent with the observations is that in liver and brain-cortex non-synaptosomal mitochondria the tyramine-oxidizing activity is distributed on both sides of the mitochondrial outer membrane, the .beta.-phenethylamine-oxidizing activity is located on the outer surface of the outer membrane and the 5-hydroxytryptamine-oxidizing activity is located on the inner surface of the mitochondrial outer membrane.