Ovine Leukocyte Microparticles Generated by the CentriMag Ventricular Assist Device In Vitro.
Ovine Leukocyte Microparticles Generated by the CentriMag Ventricular Assist Device In Vitro.
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CentriMag 心室辅助装置在体外产生的绵羊白细胞微粒。
DOI：
10.1111/aor.13068
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发表时间：
2018
影响因子：
2.4
通讯作者：
Pieper IL
中科院分区：
文献类型：
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作者：
Pieper IL
心室辅助装置（VAD）是心力衰竭患者中挽救生命的机械循环支持形式。然而，由于机械应力和异物引起的血细胞和蛋白质活化和损伤相关的副作用（血栓形成、出血、感染），VAD尚未充分发挥其潜力。VAD对白细胞影响的研究有限，但白细胞活化和损伤（包括微粒生成）可影响血栓形成和感染率。因此，目的是使用绵羊血和CentriMag VAD作为剪切应力模型，开发白细胞微粒（LMP）的流式细胞术评估。将绵羊血在CentriMag中泵送6小时，并在三种不同的泵操作条件（低流量、标准、高速）期间通过流式细胞术分析常规样品的溶血、全血细胞计数和LMP。高速条件导致血浆游离血红蛋白显著增加;总白细胞，粒细胞，单核细胞和血小板减少; CD 45 + LMP以及两种新的LMP群体增加：CD 11bbright/HLA-DR −和CD 11bdull/HLA-DR+，两者都是CD 14 −/CD 21 −。CD 11bbright/HLA-DR − LMP似乎对剪切幅度的增加有反应，而CD 11bdull/HLA-DR + LMP在所有泵送条件下均显著增加。我们提出这两个群体分别从粒细胞和T细胞中释放，但需要进一步的研究来更好地表征这两个群体。
Ventricular assist devices (VADs) are a life‐saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and proteins caused by mechanical stress and foreign materials. Studies of the effects of VADs on leukocytes are limited, yet leukocyte activation and damage including microparticle generation can influence both thrombosis and infection rates. Therefore, the aim was to develop a multicolor flow cytometry assessment of leukocyte microparticles (LMPs) using ovine blood and the CentriMag VAD as a model for shear stress. Ovine blood was pumped for 6 h in the CentriMag and regular samples analyzed for hemolysis, complete blood counts and LMP by flow cytometry during three different pump operating conditions (low flow,standard,high speed). Thehigh speedcondition caused significant increases in plasma‐free hemoglobin; decreases in total leukocytes, granulocytes, monocytes, and platelets; increases in CD45+LMPs as well as two novel LMP populations: CD11bbright/HLA‐DR−and CD11bdull/HLA‐DR+, both of which were CD14−/CD21−. CD11bbright/HLA‐DR−LMPs appeared to respond to an increase in shear magnitude whereas the CD11bdull/HLA‐DR+LMPs significantly increased in all pumping conditions. We propose that these two populations are released from granulocytes and T cells, respectively, but further research is needed to better characterize these two populations.