Transcriptional regulation of the Drosophila glial gene repo
Transcriptional regulation of the Drosophila glial gene repo
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DOI：
10.1016/j.mod.2005.01.002
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发表时间：
2005-06-01
影响因子：
2.6
通讯作者：
Jones, BW
中科院分区：
文献类型：
--
作者：
Lee, BP;Jones, BW
reversed polarity（repo）是果蝇胶质细胞命运的主要调控因子--胶质细胞缺失（gcm）的一个假定靶基因。Gcm的瞬时表达之后是repo的维持表达。在repo上游DNA中发现多个Gcm结合位点。然而，尽管repo在Gcm阳性胶质细胞中表达，但在Gcm阳性血细胞中不表达。这些观察结果表明，除了Gcm的因素是必需的repo表达。在这里，我们进行了分析的顺式调控DNA元件的回购使用lacZ报告活性在转基因胚胎。我们已经发现，一个4.2 kb的DNA区域上游的回购起始位点驱动野生型回购的表达模式。我们表明，表达依赖于多个Gcm结合位点。通过异位表达Repo，我们表明Repo可以调节自己的增强子。最后，通过系统地分析回购上游DNA片段，我们表明，表达依赖于多个元素，负责在神经胶质细胞亚群的活动，以及抑制不适当的表达在表皮。我们的研究结果表明，GCM与其他因素协同作用，以控制神经胶质细胞中的repo转录。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
reversed polarity (repo) is a putative target gene of glial cells missing (gcm), the primary regulator of glial cell fate in Drosophila. Transient expression of Gcm is followed by maintained expression of repo. Multiple Gcm binding sites are found in repo upstream DNA. However, while repo is expressed in Gcm positive glia, it is not expressed in Gcm positive hemocytes. These observations suggest factors in addition to Gcm are required for repo expression. Here we have undertaken an analysis of the cis-regulatory DNA elements of repo using lacZ reporter activity in transgenic embryos. We have found that a 4.2 kb DNA region upstream of the repo start site drives the wild-type repo expression pattern. We show that expression is dependent on multiple Gcm binding sites. By ectopically expressing Repo, we show that Repo can regulate its own enhancer. Finally, by systematically analyzing fragments of repo upstream DNA, we show that expression is dependent on multiple elements that are responsible for activity in subsets of glia, as well as repressing inappropriate expression in the epidermis. Our results suggest that Gcm acts synergistically with other factors to control repo transcription in glial cells. (c) 2005 Elsevier Ireland Ltd. All rights reserved.