Identification of a novel essential two-component signal transduction system, YhcSR, in Staphylococcus aureus
Identification of a novel essential two-component signal transduction system, YhcSR, in Staphylococcus aureus
复制标题
DOI：
10.1128/jb.187.22.7876-7880.2005
复制
发表时间：
2005-11-01
影响因子：
3.2
通讯作者：
Ji, YD
中科院分区：
文献类型：
--
作者：
Sun, JS;Zheng, L;Ji, YD
双组分信号转导系统通过感知栖息地的变化和改变基因表达，在细菌适应各种环境的能力中发挥着重要作用。在这项研究中，我们报告了金黄色葡萄球菌中的一种新型双组分系统 YhcSR，这是细菌体外生长所需的。我们发现通过诱导yhcS反义RNA下调yhcSR表达可以抑制和终止细菌生长。此外，如果没有互补的 yhcS 或 yhcR，则无法恢复可行的 yhcS 或 yhcR 基因替代突变体。总的来说，这些结果表明 YhcSR 调控系统对于金黄色葡萄球菌的培养生长是不可或缺的。此外，诱导的yhcS反义RNA选择性增加细菌对磷霉素的敏感性。这些数据表明，YhcSR 可能调节对细菌生存至关重要的基因的表达，并且可能是开发新型抗菌剂的潜在目标。
Two-component signal transduction systems play an important role in the ability of bacteria to adapt to various environments by sensing changes in their habitat and by altering gene expression. In this study, we report a novel two-component system, YhcSR, in Staphylococcus aureus which is required for bacterial growth in vitro. We found that the down-regulation of yhcSR expression by induced yhcS antisense RNA can inhibit and terminate bacterial growth. Moreover, without complementary yhcS or yhcR, no viable yhcS or yhcR gene replacement mutant was recoverable. Collectively, these results demonstrated that the YhcSR regulatory system is indispensable for S. aureus growth in culture. Moreover, induced yhcS antisense RNA selectively increased bacterial susceptibility to phosphomycin. These data suggest that YhcSR probably modulates the expression of genes critical for bacterial survival and may be a potential target for the development of novel antibacterial agents.