DIRECT FLUORESCENCE MEASUREMENT OF DIFFUSIONAL WATER PERMEABILITY IN THE VASOPRESSIN-SENSITIVE KIDNEY COLLECTING TUBULE
DIRECT FLUORESCENCE MEASUREMENT OF DIFFUSIONAL WATER PERMEABILITY IN THE VASOPRESSIN-SENSITIVE KIDNEY COLLECTING TUBULE
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DOI：
10.1016/s0006-3495(88)82993-x
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发表时间：
1988-10-01
影响因子：
3.4
通讯作者：
VERKMAN, AS
中科院分区：
文献类型：
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作者：
KUWAHARA, M;VERKMAN, AS
基于荧光团氨基萘三磺酸（ANTS）对溶液中H2O/D2 O含量的敏感性，建立了一种快速、准确测定灌流肾小管跨上皮扩散水渗透性（Pd）的荧光方法。ANTS的荧光在H2O缓冲液中比在D2 O缓冲液中低3.2倍。ANTS荧光对溶液中H2O/D2 O含量变化的响应发生在530个发射波长处。根据小管几何形状、管腔流量、ANTS荧光和ANTS荧光与H2O/D2 O校准关系测定Pd。浴H2O/D2 O含量变化的仪器响应时间为
A fluorescence method has been developed for accurate instantaneous measurement of transepithelial diffusional water permeability (Pd) in perfused kidney tubules based on the sensitivity of the fluorophore aminonapthelane trisulfonic acid (ANTS) to solution H2O/D2O content. The fluorescence of ANTS was 3.2-fold lower in an H2O buffer than in a D2O buffer. The response of ANTS fluorescence to a change in solution H2O/D2O content occurred in 530 emission wavelengths. Pd was determined from tubule geometry, lumen flow, ANTS fluoresences, and ANTS fluorescence vs. H2O/D2O calibration relation. The instrument response time for a change in bath H2O/D2O content was