Arabinogalactan glycoprotein dynamics during the progamic phase in the tomato pistil
Arabinogalactan glycoprotein dynamics during the progamic phase in the tomato pistil
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DOI：
10.1007/s00497-021-00408-1
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发表时间：
2021-04
影响因子：
3.4
通讯作者：
Cecilia M Lara-Mondragón;Cora A. MacAlister
中科院分区：
文献类型：
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作者：
Cecilia M Lara-Mondragón;Cora A. MacAlister
关键信息雌蕊AGPs显示动态定位模式，在番茄受精。SlyFLA 9（Solyc07g065540.1）是一个嵌合的Fasciclin-like AGP，在子房中表达丰富，在花粉-雌蕊相互作用中具有潜在的功能。阿拉伯半乳聚糖糖蛋白（AGPs）是一类高度糖基化的分泌蛋白，广泛参与植物的生殖过程，特别是雌蕊内的生殖过程。虽然番茄（Solanum lycopersicum）是一种重要的作物，需要成功的施肥生产，这一事件的分子基础仍然研究不足。在这里，我们探索的时空定位的AGP在成熟的番茄雌蕊受精前后。使用组织学技术检测AGP糖部分，我们发现，积累的AGP与成熟的柱头，我们确定了一个AGP亚群限制在受精后不再可见的珠孔。为了确定候选雌蕊AGP基因，我们使用了RNA测序的方法来编目成熟番茄雌蕊（柱头，顶端和基部花柱和子房）以及花粉和花粉管的功能不同的子部分中的基因表达。在番茄基因组中编码的161个预测的AGP和AGP样蛋白中，我们确定了4个在生殖组织中特异性富集表达的基因。我们进一步验证了其中两个的表达，Fasciclin-like AGP（SlyFLA 9，Solyc07g065540.1）和新的杂交AGP（SlyHAE，Solyc09g075580.1）。原位杂交结果表明，SlyFLA 9在胚珠珠被和果皮中均有表达。此外，雌蕊转录组的差异表达分析揭示了以前未报道的基因在成熟雌蕊的每个子节中富集表达，为未来的功能研究奠定了基础。
Key messagePistil AGPs display dynamic localization patterns in response to fertilization in tomato. SlyFLA9 (Solyc07g065540.1) is a chimeric Fasciclin-like AGP with enriched expression in the ovary, suggesting a potential function during pollen–pistil interaction.AbstractDuring fertilization, the male gametes are delivered by pollen tubes to receptive ovules, deeply embedded in the sporophytic tissues of the pistil. Arabinogalactan glycoproteins (AGPs) are a diverse family of highly glycosylated, secreted proteins which have been widely implicated in plant reproduction, particularly within the pistil. Though tomato (Solanum lycopersicum) is an important crop requiring successful fertilization for production, the molecular basis of this event remains understudied. Here we explore the spatiotemporal localization of AGPs in the mature tomato pistil before and after fertilization. Using histological techniques to detect AGP sugar moieties, we found that accumulation of AGPs correlated with the maturation of the stigma and we identified an AGP subpopulation restricted to the micropyle that was no longer visible upon fertilization. To identify candidate pistil AGP genes, we used an RNA-sequencing approach to catalog gene expression in functionally distinct subsections of the mature tomato pistil (the stigma, apical and basal style and ovary) as well as pollen and pollen tubes. Of 161 predicted AGP and AGP-like proteins encoded in the tomato genome, we identified four genes with specifically enriched expression in reproductive tissues. We further validated expression of two of these, a Fasciclin-like AGP (SlyFLA9,Solyc07g065540.1) and a novel hybrid AGP (SlyHAE,Solyc09g075580.1). Using in situ hybridization, we also foundSlyFLA9was expressed in the integuments of the ovule and the pericarp. Additionally, differential expression analyses of the pistil transcriptome revealed previously unreported genes with enriched expression in each subsection of the mature pistil, setting the foundation for future functional studies.