Enzymatic pathway for biodegrading microcystin LR in Sphingopyxis sp C-1
Enzymatic pathway for biodegrading microcystin LR in Sphingopyxis sp C-1
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DOI：
10.1016/j.jbiosc.2012.07.004
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Sugiura, Norio
中科院分区：
文献类型：
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作者：
Shimizu, Kazuya;Maseda, Hideaki;Sugiura, Norio
由mlrA、mlrB、mlrC和mlrD组成的Ink基因簇参与了蓝藻毒素微囊藻毒素的降解。然而，目前还不清楚哪些降解中间产物是由MlrB和MlrC代谢的。为了解决这些问题，我们构建了重组大肠杆菌来高效表达MlrB和MlrC。C-1，并测定了哪些中间体在无细胞提取物中被降解。含有MlrB的无细胞提取物降解线性化的微囊藻毒素-LR，产生四肽。MlrC的无细胞提取液可降解线性化的微囊藻毒素-LR，也可将四肽降解为氨基酸ADDA。这些结果表明，MlrB和MlrC都能降解线性化的微囊藻毒素-LR，而MlrC能专一性地降解四肽。C-1。(C)2012年，日本生物技术学会。版权所有。
The Ink gene cluster consisting of mlrA, mlrB, mlrC, and mlrD is involved in the degradation of the cyanobacterial toxin microcystin. However, it is unclear which degradation intermediates are metabolized by MlrB and MlrC. To address these questions, we constructed recombinant Escherichia coli to overproduce MlrB and MlrC from Sphingopyxis sp. C-1, and determined which intermediates were degraded in cell-free extracts. The cell-free extract containing MlrB degraded linearized microcystin-LR, giving rise to a tetrapeptide. The cell-free extract of MlrC degraded linearized microcystin-LR and also degraded the tetrapeptide to the amino acid Adda. These results indicate that linearized microcystin-LR is degraded by both MlrB and MlrC, and tetrapeptide is degraded by specifically by MlrC in Sphingopyxis sp. C-1. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.