UCHP Method for Oligosaccharide Combinatorial Library Synthesis
UCHP Method for Oligosaccharide Combinatorial Library Synthesis
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DOI：
10.1080/07328300903100661
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发表时间：
2009-01-01
影响因子：
1
通讯作者：
Machida, Sachiko
中科院分区：
文献类型：
--
作者：
Komba, Shiro;Machida, Sachiko
开发了一种使用特殊羟基保护基团（单化学羟基保护（UCHP）基团）进行寡糖组合文库合成的新方法。 UCHP基团由寡聚氨基酸衍生物组成。 UCHP 基团的氨基末端受到 Boc 或 Fmoc 基团的保护。利用这两种类型的UCHP，在固体支持物上成功合成了五种三半乳糖[Gal beta 1-3Gal beta 1-3Gal、Gal beta 1-3(Gal beta 1-4) Gal、Gal beta 1-4Gal beta 1-3Gal、Gal beta 1-3Gal beta 1-4Gal和Gal beta 1-4Gal beta 1-4Gal]作为寡糖的模型组合库。所有反应的每个步骤也通过结合使用两种比色测试（四氯苯醌和甲基红-DIC）成功监测。
A new methodology for oligosaccharide combinatorial library synthesis using a special hydroxy protecting group, the uni-chemo hydroxy protection (UCHP) group, was developed. The UCHP group was composed of oligomeric amino acid derivatives. The amino terminals of UCHP groups were protected by either Boc or Fmoc groups. By using these two types of UCHP, five kinds of trigalactoses [Gal beta 1-3Gal beta 1-3Gal, Gal beta 1-3(Gal beta 1-4) Gal, Gal beta 1-4Gal beta 1-3Gal, Gal beta 1-3Gal beta 1-4Gal, and Gal beta 1-4Gal beta 1-4Gal] were successfully synthesized on a solid support as a model of oligosaccharide combinatorial library. Each step of all reactions was also successfully monitored using a combination of two colorimetric tests, chloranil and methyl red-DIC.