Molecular identificationofsterolregulatory element‐bindingprotein‐1cfromHelicoverpa armigerapheromone gland
Molecular identificationofsterolregulatory element‐bindingprotein‐1cfromHelicoverpa armigerapheromone gland
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棉铃虫信息素腺固醇调节元件结合蛋白1c的分子鉴定
DOI：
10.1002/arch.21505
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发表时间：
2018
影响因子：
2.2
通讯作者：
An Shiheng
中科院分区：
文献类型：
--
作者：
Zhang Yunhui;Ma Nana;Yin Letong;Wei Jizhen;Xi Yuqiang;Zhao Xincheng;Du Mengfang;An Shiheng
固醇调节元件结合蛋白-1c(SREBP-1c)是一种基本的螺旋-环-螺旋型转录因子，调节脂肪酸和胆固醇的生物合成。鳞翅目性信息素是脂肪酸生物合成和碳链修饰的产物。然而，SREBP-1c在性信息素生物合成中的作用仍然不清楚。本研究以棉铃虫为模型，研究了SREBP-1性信息素生物合成(HaSREBP-1c)的作用。序列分析表明，HaSREBP-1c的开放阅读框由3201个 碱基组成，编码1066个氨基酸残基。基于氨基酸序列的BLAST搜索表明，HaSREBP-1c与鳞翅目昆虫的同源物有更高的氨基酸同源性。发育过程中的表达谱显示，成虫羽化前72 小时可检测到HaSREBP-1转录产物，然后逐渐增加，成虫羽化后24 小时达到高峰。空间表达模式表明，HaSREBP-1c在所有受检组织中普遍表达。RNA干扰显示，HaSREBP-1cMessenger RNA(MRNA)水平的降低导致乙酰辅酶A羧基酶(ACC)mRNA水平和随之而来的性信息素产生显著下降。行为学分析表明，HaSREBP-1cmRNA水平的降低导致雌性吸引雄性的能力显著下降。综上所述，我们的结果表明HaSREBP-1cas作为一个转录因子来调节ACC基因的表达，从而影响雌虫性信息素的生物合成。
Sterol regulatory element‐binding protein‐1c (SREBP‐1c) is a basic helix‐loop‐helix type transcription factor that regulates the fatty acid and cholesterol biosynthesis. Lepidoptera sex pheromone is a product of fatty acid biosynthesis followed by carbon chain modifications. However, the role of SREBP‐1c on sex pheromone biosynthesis remains elusive. In the present study,Helicoverpa armigerawas used as a model to investigate the role ofSREBP‐1con sex pheromone biosynthesis (HaSREBP‐1c). Sequence analysis demonstrated that the open reading frame ofHaSREBP‐1cconsists of 3201 bp nucleotides that encode 1066 amino acid residues. Blast searches based on amino acid sequences demonstrated that HaSREBP‐1c shares higher amino acid identity with lepidopteran homologues. Development expression profiles demonstrated thatHaSREBP‐1ctranscript could be detected at 72 hr before adult emergence, then gradually increased, and finally reached its peak at 24 hr after adult emergence. The spatial expression pattern demonstrated thatHaSREBP‐1cwas ubiquitously expressed in all examined tissues. The decrease ofHaSREBP‐1cmessenger RNA (mRNA) levels as shown by RNA interference caused significant decreases in acetyl‐coA carboxylase (ACC) mRNA levels and subsequent sex pheromone production. Behavior analysis demonstrated that the decrease ofHaSREBP‐1cmRNA level caused a significant decrease in the female’s ability to attract males. Altogether our results demonstrated thatHaSREBP‐1cacts as a transcription factor to regulate ACC mRNA expression and, therefore, to influence female sex pheromone biosynthesis.