Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes
Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Cells and Pericytes
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DOI：
10.1007/978-1-62703-604-7_2
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Stratman, Amber N.
中科院分区：
文献类型：
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作者：
Davis, George E.;Kim, Dae Joong;Stratman, Amber N.
使用体外EC管形态发生和成熟模型的一个重要进展是使用无血清限定培养基的系统的开发。使用这种方法，可以确定这些事件实际上所需的生长因子和细胞因子。我们实验室开发的第一个模型是这样一个系统，其中我们表明需要佛波酯来促进3D胶原基质中的存活和管形态发生。最近，我们开发了一种新的系统，其中加入造血干细胞细胞因子、干细胞因子（SCF）、白细胞介素-3（IL-3）和基质衍生因子-1 α（SDF-1 α）与FGF-2，以促进在无血清限定条件下在3D胶原基质中的人EC管形态发生。这种使用SCF、IL-3、SDF-1 α和FGF-2的新模型在加入周细胞后也工作良好，其中EC管形成发生，周细胞被募集到管中，并且在EC-周细胞相互作用后发生血管基底膜基质组装。在这一章中，我们描述了几个体外试验模型，我们经常利用调查的分子要求，EC管的形成和成熟事件在三维细胞外基质环境中是至关重要的。
An important advance using in vitro EC tube morphogenesis and maturation models has been the development of systems using serum-free defined media. Using this approach, the growth factors and cytokines which are actually necessary for these events can be determined. The first model developed by our laboratory was such a system where we showed that phorbol ester was needed in order to promote survival and tube morphogenesis in 3D collagen matrices. Recently, we have developed a new system in which the hematopoietic stem cell cytokines, stem cell factor (SCF), interleukin-3 (IL-3), and stromal derived factor-1 alpha (SDF-1 alpha) were added in conjunction with FGF-2 to promote human EC tube morphogenesis in 3D collagen matrices under serum-free defined conditions. This new model using SCF, IL-3, SDF-1 alpha, and FGF-2 also works well following the addition of pericytes where EC tube formation occurs, pericytes are recruited to the tubes, and vascular basement membrane matrix assembly occurs following EC-pericyte interactions. In this chapter, we describe several in vitro assay models that we routinely utilize to investigate the molecular requirements that are critical to EC tube formation and maturation events in 3D extracellular matrix environments.