Sequence and functional characterization of feline interleukin 2.
Sequence and functional characterization of feline interleukin 2.
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猫白介素 2 的序列和功能表征。
DOI：
10.1006/bbrc.1993.1926
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发表时间：
1993
影响因子：
3.1
通讯作者：
Leff,AR
中科院分区：
文献类型：
--
作者：
Cozzi,PJ;Padrid,PA;Takeda,J;Alegre,ML;Yuhki,N;Leff,AR
自从发现其参与猫免疫缺陷病毒感染（“猫AIDS”）和猫白血病病毒感染的发病机制以来，猫白细胞介素2（IL-2）的作用一直是特别感兴趣的焦点。本研究的目的是克隆猫IL-2 cDNA，并合成具有生物活性的重组猫IL-2。采用PCR方法和猫白细胞cDNA文库筛选技术，分离编码猫IL-2的cDNA。猫IL-2由154个氨基酸组成，包括一个推定的信号序列，与人、牛、鼠和大鼠IL-2分别具有81%、69%、60%和64%的同一性。猫IL-2 cDNA在COS-7细胞中表达。所分泌的蛋白质具有真正IL-2特征性的CTLL-4鼠细胞毒性T细胞增殖活性。
Since the discovery of its involvement in the pathogenesis of feline immunodeficiency virus infection ("cat AIDS") and feline leukemia virus infection, the role of feline interleukin 2 (IL-2) has been a focus of particular interest. The purpose of this study was to clone feline IL-2 cDNA, as well as synthesize bioactive recombinant feline IL-2. The isolation of cDNA encoding feline IL-2 was carried out using a PCR-based strategy and screening of a feline leukocyte cDNA library. Feline IL-2 consists of 154 amino acids including a putative signal sequence and has 81%, 69%, 60% and 64% identity to human, bovine, murine and rat IL-2, respectively. Feline IL-2 cDNA was expressed in COS-7 cells. The secreted protein has CTLL-4 murine cytotoxic T cell proliferative activity characteristic of authentic IL-2.