A novel carotenoid 1,2-hydratase (CruF) from two species of the non-photosynthetic bacterium Deinococcus
A novel carotenoid 1,2-hydratase (CruF) from two species of the non-photosynthetic bacterium Deinococcus
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来自两种非光合细菌奇球菌的新型类胡萝卜素 1,2-水合酶 (CruF)
DOI：
10.1099/mic.0.027623-0
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发表时间：
2009-08-01
期刊：
影响因子：
2.8
通讯作者：
Hua, Yuejin
中科院分区：
文献类型：
--
作者：
Sun, Zongtao;Shen, Shaochuan;Hua, Yuejin
在非光合细菌Deinococcus radiodurans R1和Deinococcus geothermalis DSM 11300中鉴定了一种负责γ-胡萝卜素C-1 '，2'水合的新型类胡萝卜素1，2-水合酶（CruF）。基因表达和破坏实验表明，dr 0091和dgeo 2309编码D. radiodurans和D. geothermalis，分别。它们的同源物也被发现在蓝藻的基因组中，并表现出很少的同源性，主要是在光合细菌中发现的羟基神经孢烯合酶（CrtC）蛋白。系统发育分析表明，CruF同源物形成一个独立的家庭，这是从已知的CrtC家庭的进化距离。
A novel carotenoid 1,2-hydratase (CruF) responsible for the C-1',2' hydration of gamma-carotene was identified in the non-photosynthetic bacteria Deinococcus radiodurans R1 and Deinococcus geothermalis DSM 11300. Gene expression and disruption experiments demonstrated that dr0091 and dgeo2309 encode CruF in D. radiodurans and D. geothermalis, respectively. Their homologues were also found in the genomes of cyanobacteria, and exhibited little homology to the hydroxyneurosporene synthase (CrtC) proteins found mainly in photosynthetic bacteria. Phylogenetic analysis showed that CruF homologues form a separate family, which is evolutionarily distant from the known CrtC family.