Y Chromosome DNA in Women's Vaginal Samples as a Biomarker of Recent Vaginal Sex and Condom Use With Male Partners in the HPV Infection and Transmission Among Couples Through Heterosexual Activity Cohort Study.
Y Chromosome DNA in Women's Vaginal Samples as a Biomarker of Recent Vaginal Sex and Condom Use With Male Partners in the HPV Infection and Transmission Among Couples Through Heterosexual Activity Cohort Study.
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DOI：
10.1097/olq.0000000000000688
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发表时间：
2018-01
影响因子：
3.1
通讯作者：
HITCH study group
中科院分区：
文献类型：
--
作者：
Malagón T;Burchell A;El-Zein M;Guénoun J;Tellier PP;Coutlée F;Franco EL;HITCH study group
在实验研究中，在无保护性行为后的阴道样本中检测到来自男性上皮细胞和精子细胞的Y染色体DNA。我们在一项观察性研究中评估了这种关联的强度，以检验Y染色体DNA在流行病学研究中作为近期性行为生物标志物的实用性。HITCH队列研究招募了2005-2010年在加拿大蒙特利尔大学或学院就读的502名女性及其男性伴侣。参与者完成了自填问卷。我们使用实时PCR检测女性基线阴道样本的Y染色体DNA，并评估哪些性行为是Y染色体DNA阳性和数量的独立预测因子，采用logistic和负二项回归。Y染色体DNA阳性率从0-1天前报告阴道性行为的伴侣中的77%下降到最近一次阴道性行为>=15天前报告阴道性行为的伴侣中的13%（校正比值比=0.09，95%CI：0.02-0.36）。阴道脱落细胞中Y染色体DNA的平均比例在经常使用避孕套的女性中（0.01%）远低于从不使用避孕套的女性（2.07%）（校正比26.8，95%CI：8.9-80.5）。没有发现与报告的口头/数字性行为频率或伴侣关系的并发性相关。Y染色体DNA数量与上次阴道性交后的天数以及观察环境中是否使用避孕套密切相关。在缺乏性行为数据的观察性研究中，Y染色体DNA数量可能被证明是最近阴道性行为的相关因素，例如人乳头瘤病毒感染流行率的监测研究。
Y chromosome DNA from male epithelial and sperm cells a detected in vaginal samples after unprotected sex in experimental studies. We assessed the strength of this association in an observational setting to examine the utility of Y chromosome DNA as a biomarker of recent sexual behaviors in epidemiological studies. The HITCH cohort study enrolled 502 women attending a university or college in Montréal, Canada, and their male partners from 2005–2010. Participants completed self-administered questionnaires. We used real-time PCR to test women’s baseline vaginal samples for Y chromosome DNA, and assessed which sexual behaviors were independent predictors of Y chromosome DNA positivity and quantity with logistic and negative binomial regression. Y chromosome DNA positivity decreased from 77% in women in partnerships reporting vaginal sex 0–1 days ago to 13% in women in partnerships reporting last vaginal sex >=15 days ago (adjusted odds ratio=0.09, 95%CI: 0.02–0.36). The mean proportion of exfoliated vaginal sample cells with Y chromosome DNA was much lower for women who reported always using condoms (0.01%) than for women who reported never using condoms (2.07%) (adjusted ratio 26.8, 95%CI: 8.9–80.5). No association was found with reported oral/digital sex frequency or concurrency of partnerships. Y chromosome DNA quantity is strongly associated with days since last vaginal sex and lack of condom use in observational settings. Y chromosome DNA quantity may prove useful as a correlate of recent vaginal sex in observational studies lacking data on sexual behavior, such as surveillance studies of human papillomavirus infection prevalence.