Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles.
Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles.
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胞浆内单精子注射的成功率高于带下授精。
作者：
A. V. Van Steirteghem;Jiaen Liu;H. Joris;Z. Nagy;C. Janssenswillen;H. Tournaye;M. Derde;E. van Assche;P. Devroey
对无法通过常规体外受精治疗的夫妇进行了300个治疗周期的亚带性人工授精(SUZI)和卵胞浆内单精子注射(ICSI)。ICSI的卵母细胞受损率(13.5%)明显高于苏子(7.1%)。ICSI后受精率(51.0%)显著高于苏子后(14.3%)，且与精液性质有关。两种方法的卵裂率相似(77%)。共移植胚胎217个(占治疗周期的72.3%)，妊娠66个，妊娠率分别为22.0%和30.4%。到目前为止，27.3%的孕妇发生了妊娠丢失，9名健康儿童在8次分娩后出生，41例临床妊娠进展顺利。对35例孕妇进行了绒毛取样或羊膜穿刺术，经细胞遗传学分析，获得39例正常胎儿核型。
Subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) were carried out in 300 treatment cycles in couples unable to be helped by conventional in-vitro fertilization treatment. More oocytes were damaged by ICSI (13.5%) than by SUZI (7.1%). The normal fertilization rate was substantially higher after ICSI (51.0%) than after SUZI (14.3%) and was related to the semen characteristics. The cleavage rate was similar for both procedures (77%). After 217 embryo transfers (72.3% of the treatment cycles) 66 pregnancies were established, i.e. pregnancy rates of 22.0% per started cycle and 30.4% per embryo transfer. So far, pregnancy loss has occurred in 27.3% of the pregnancies, nine healthy children have been born after eight deliveries and 41 clinical pregnancies are progressing uneventfully. Chorionic villus sampling or amniocentesis have been performed in 35 pregnancies and 39 normal fetal karyotypes have been obtained after cytogenetic analysis.