Comparison between the characteristics of follicular fluid and the developmental competence of bovine oocytes
Comparison between the characteristics of follicular fluid and the developmental competence of bovine oocytes
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DOI：
10.1016/j.anireprosci.2005.04.006
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发表时间：
2006-02-01
影响因子：
2.2
通讯作者：
Monji, Y
中科院分区：
文献类型：
--
作者：
Iwata, H;Inoue, J;Monji, Y
从奶牛个体采集的卵母细胞的发育能力存在很大差异。卵母细胞在卵泡液中生长和成熟。在本研究中，调查了每个卵巢的卵泡发育特性和封闭卵母细胞的发育能力，并对这些数据进行了比较。共采集了37对小母牛的卵巢。血清镁(Mg)、门冬氨酸氨基转移酶(AST)、非酯化脂肪酸(NEFA)、乳酸脱氢酶(LDH)均高于血清标准。在这些特征之间发现了几个显著的相关性。受精后48小时，胚胎发育后期阶段(6细胞期)与受精后8天的囊胚率有关。此外，胚胎发育能力(胚胎卵裂率和囊胚率)与卵黄指数(ICT)和血尿素氮(BUN)浓度呈显著正相关或显著负相关。综上所述，卵母细胞的质量受卵泡内环境的影响，BUN或ICT是预测卵母细胞发育能力的指标。(C)2005 Elsevier B.V.保留所有权利。
There are great differences in the developmental competence of oocytes collected from individual cows. Oocytes grow and mature in the follicular fluid (FF). In the present study, characteristics of the FF of each ovary and the developmental competence of enclosed oocytes were investigated, and these data were then compared. A total of 37 pairs of ovaries were collected from beef heifers. The concentration of magnesium (Mg), aspirate aminotransferase (AST), nonesterified fatty acids (NEFA), and lactate dehydrogenase (LDH) in the FF were great compared with serum standard. Several significant correlations among these characteristics were detected. Forty-eight hours after fertilization, the stage of embryo development at an advanced developmental stage (>6 cell stage) is related to the rate of blastulation 8 days after fertilization. In addition, a significantly positive or negative correlation was observed between the developmental competence (the rate of cleavage in the embryo and blastulation) and the concentration of the icterus index (ICT) or blood urea nitrogen (BUN) in the FF. In conclusion, the quality of oocytes is affected by the environment in the follicle, and BUN or ICT is a predictable index of the developmental competence of oocytes. (c) 2005 Elsevier B.V. All rights reserved.