The Drosophila ebony gene is closely related to microbial peptide synthetases and shows specific cuticle and nervous system expression
The Drosophila ebony gene is closely related to microbial peptide synthetases and shows specific cuticle and nervous system expression
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DOI：
10.1016/s0378-1119(98)00440-5
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发表时间：
1998-10-09
期刊：
影响因子：
3.5
通讯作者：
Dessen, E
中科院分区：
文献类型：
--
作者：
Hovemann, BT;Ryseck, RP;Dessen, E
先前检测到的乌木（e）基因座（Caizzi等人，1987）由一个复杂的基因结构组成，分为七个外显子。编码98.5 kDa的推定Ebony蛋白的开放阅读框显示与肽合成酶家族的同源性（Stachelhaus和Marahiel，1995），与提出的作为β-丙氨酰-多巴胺合成酶的功能具有良好的相关性。在整个发育过程中检测到多个乌木转录本。P因子介导的基因组DNA转化拯救了角质层、电生理和行为表型。乌木阅读框架与E.大肠杆菌中表达，在表皮和神经系统中表达。在第一个和第二个视神经丛中的强染色可能反映了在乌木突变体中观察到的明显视觉缺陷。此外，在果蝇中检测到弱的中枢脑和胸神经节表达。保守的多结构域蛋白质结构已知肽合成酶应具有功能上的影响，推定的反应机制的肽键形成。(C)1998 Elsevier Science B. V.保留所有权利。
The previously detected ebony (e) locus (Caizzi et al., 1987) consists of a complex gene structure that is divided into seven exons. An open reading frame encoding the putative Ebony protein of 98.5 kDa exhibits homology to a family of peptide synthetases (Stachelhaus and Marahiel, 1995), in good correlation with the proposed function as P-alanyl-dopamine synthetase. Multiple ebony transcripts are detected throughout development. P-factor mediated transformation of genomic DNA rescues the cuticle, electrophysiological and behavioural phenotypes. Fusion of the ebony reading frame with that of P-galactosidase of E. coli reveals expression in cuticle and nervous system. Strong staining in the first and, to a lesser extent, in the second optic neuropile may reflect the pronounced visual defect observed in ebony mutants. In addition, weak central brain and thoracic ganglion expression is detected in flies. Conservation of a multidomain protein structure known from peptide synthetases should have functional implications on the putative reaction mechanism of peptide bond formation. (C) 1998 Elsevier Science B.V. All rights reserved.