THE RELEASE OF N-ACETYLNEURAMINIC AND N-GLYCOLLOYL-NEURAMINIC ACID FROM SOLUBLE COMPLEX CARBOHYDRATES AND ERYTHROCYTES BY BACTERIAL, VIRAL AND MAMMALIAN SIALIDASES
THE RELEASE OF N-ACETYLNEURAMINIC AND N-GLYCOLLOYL-NEURAMINIC ACID FROM SOLUBLE COMPLEX CARBOHYDRATES AND ERYTHROCYTES BY BACTERIAL, VIRAL AND MAMMALIAN SIALIDASES
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DOI：
10.1042/bj1970293
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
SCHAUER, R
中科院分区：
文献类型：
--
作者：
CORFIELD, AP;VEH, RW;SCHAUER, R
制备了一系列底物：唾液酸(2→fwdarw.3)乳糖、唾液酸(2→fwdarw.6)GalNAc和神经节苷脂GM3，其含有N-乙酰神经氨酸(AcNeu)或N-乙醇酰神经氨酸(GcNeu)。通过臭氧分解GcNeu-GM3制备三糖GcNeu(2.fwdarw.3)乳糖，通过碱消除从牛颌下腺粘蛋白中分离二糖AcNeu(2.fwdarw.6)GalNAc和GcNeu(2.fwdarw.6)GalNAc。使用上述底物对来自新城疫病毒、鸡瘟病毒、流感病毒 A2、产气荚膜梭菌、霍乱弧菌、尿素节杆菌和人肝溶酶体的唾液酸酶进行了研究，与相应的含 AcNeu 的化合物相比，所有含 GcNeu 的底物均显示出较差的裂解能力。这反映在这些唾液酸酶的 Km is Vmax 值中。病毒和细菌唾液酸酶之间的差异可以根据唾液酸释放的动力学常数和时间曲线来检测。还用牛颌下腺粘蛋白以及人和猪红细胞的混合物、含有AcNeu和GcNeu的大分子底物观察到AcNeu相对于GcNeu的优先释放。唾液酸酶对 AcNeu 和 GcNeu 差异裂解的重要性与 GcNeu 在生理系统中的作用的例子一起考虑。
A series of substrates, sialyl(2 .fwdarw. 3)lactose, sialyl(2 .fwdarw. 6)GalNAc and ganglioside GM3, containing either N-acetylneuraminic acid (AcNeu) or N-glycolloylneuraminic acid (GcNeu), was prepared. The trisaccharide GcNeu(2 .fwdarw. 3)lactose was prepared by ozonolysis of GcNeu-GM3 and the disaccharides AcNeu(2 .fwdarw. 6)GalNAc and GcNeu(2 .fwdarw. 6)GalNAc were isolated from bovine submandibular-gland mucin by alkali elimination. Sialidases from Newcastle-disease virus, fowl-plague virus, influenza virus A2, Clostridium perfringens, Vibrio cholerae, Arthrobacter ureafaciens and human liver lysosomes were studied with the above substrates and all showed poorer cleavage of GcNeu-containing substrates when compared with the corresponding AcNeu-containing compounds. This was reflected in the Km was Vmax values of these sialidases. Differences between viral and bacterial sialidases could be detected on the basis of their kinetic constants and time curves of sialic acid release. Preferred release of AcNeu relative to GcNeu was also observed with bovine submandibular gland mucin and a mixture of human and porcine erythrocytes, macromolecular substrates containing both AcNeu and GcNeu. The significance of differential cleavage of AcNeu and GcNeu by sialidases is considered together with examples of the role of GcNeu in physiological systems.