Green synthesis of carbon quantum dots from plant turmeric holds promise as novel photosensitizer for in vitro photodynamic antimicrobial activity
Green synthesis of carbon quantum dots from plant turmeric holds promise as novel photosensitizer for in vitro photodynamic antimicrobial activity
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DOI：
10.1016/j.jmrt.2022.11.090
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发表时间：
2022-11
期刊：
影响因子：
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通讯作者：
Hongjun Yan;Peiyuan Li;Fangzhou Wen;Qiuting Xu;Qing Guo;Wei Su
中科院分区：
文献类型：
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作者：
Hongjun Yan;Peiyuan Li;Fangzhou Wen;Qiuting Xu;Qing Guo;Wei Su
研究表明，碳量子点（CQDs）由于其优异的物理化学性质，在光动力学治疗中作为一种新型的碳纳米材料具有很高的活性。然而，以前昂贵的前体和耗时的生产工艺，以及复杂的掺杂/官能化形式限制了它们的经济设计和使用。本研究以天然植物姜黄为碳源，采用简单、绿色的方法制备了ST-JHCQDs，并对其进行了理化和光学表征。大肠杆菌和S.在蓝光照射下，金红色的形成取决于炭化程度、浓度和光照时间。生物分子渗漏和激光共聚焦扫描显微镜分析表明，ST-JHCQDs在蓝光照射下有效地产生活性氧（ROS），这导致两种细菌的细胞膜完整性和细胞内大分子渗漏的干扰。同时，扫描电子显微镜图像显示细胞膜破裂和碎片化，这与ROS损伤一致，证明了ST-JHCQDs作为光敏剂在体外光动力学抗微生物活性中的有效性。这些实验表明，姜黄素CQDs是一种具有巨大抗菌潜力的新型天然光敏剂材料。
Research has shown that carbon quantum dots (CQDs) are active as novel carbon nanomaterials in photodynamic therapy due to their excellent photophysical properties. However, previously expensive precursors and time-consuming production processes, as well as complex doping/functionalization forms have limited their economic design and use. In this study, we prepared ST-JHCQDs by a simple and green method using natural plant turmeric as the carbon source, and characterized ST-JHCQDs by physical and optical means.In vitroantibacterial results showed that the antibacterial effects of ST-JHCQDs againstE. coliandS. aureusunder the irradiation of blue light depended on carbonization degrees, concentration and light duration. Biomolecule leakage and confocal laser scanning microscope analysis showed that ST-JHCQDs were effective in producing reactive oxygen species (ROS) under blue light irradiation, which resulted in disturbance of cell membrane integrity and leakage of intracellular macromolecules in both bacteria. Meanwhile, scanning electron microscopy images showed cell membrane wrinkling and fragmentation, which was consistent with ROS damage, demonstrating the effectiveness of ST-JHCQDs as photosensitizersin vitrophotodynamic antimicrobial activity. These experiments show that CQDs prepared from turmeric was a new natural photosensitizer material with great antibacterial potential.