Fabrication of a nanocarrier system through self-assembly of plasma protein and its tumor targeting
Fabrication of a nanocarrier system through self-assembly of plasma protein and its tumor targeting
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DOI：
10.1088/0957-4484/22/29/295603
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发表时间：
2011-07-22
期刊：
影响因子：
3.5
通讯作者：
Hu, Yiqiao
中科院分区：
文献类型：
--
作者：
Gong, Guangming;Zhi, Feng;Hu, Yiqiao
人血清白蛋白（HSA）纳米粒作为靶向给药的纳米载体系统具有很大的前景。本研究的目的是探索使用二硫键断裂剂β-巯基乙醇（β-ME）制备尺寸可控的白蛋白纳米粒的可能性。结果表明，蛋白质浓度和温度对白蛋白纳米颗粒的大小有积极的影响，而pH值对纳米颗粒的形成速率有负面影响。β-ME的加入引起HSA二级结构的变化，并暴露HSA的疏水核心，导致纳米颗粒的形成。人血清白蛋白纳米粒可被MCF-7细胞内化，主要聚集在细胞浆中。在荷瘤小鼠中注射后，HSA纳米颗粒在肿瘤组织中积累，证明了纳米颗粒的靶向能力。因此，人血清白蛋白可以通过断裂二硫键而制成纳米粒，并且这些纳米粒显示出高的肿瘤靶向能力。人血清白蛋白纳米粒可能是理想的靶向递送药物活性物质。
Human serum albumin (HSA) nanoparticles hold great promise as a nanocarrier system for targeted drug delivery. The objective of this study was to explore the possibility of preparing size controllable albumin nanoparticles using the disulfide bond breaking reagent beta-mercaptoethanol (beta-ME). The results showed that the protein concentration and temperature had positive effects on the sizes of the albumin nanoparticles, while pH had a negative effect on the rate of nanoparticle formation. The addition of beta-ME induced changes in HSA secondary structure and exposed the hydrophobic core of HSA, leading to the formation of nanoparticles. Human serum albumin nanoparticles could be internalized by MCF-7 cells and mainly accumulated in cytoplasm. After injection in tumor bearing mice, the HSA nanoparticles accumulated in tumor tissues, demonstrating the targeting ability of the nanoparticles. Therefore, human serum albumin can be fabricated into nanoparticles by breaking the disulfide bonds and these nanoparticles exhibit high tumor targeting ability. Human serum albumin nanoparticles could be ideal for the targeted delivery of pharmacologically active substances.