Requirement for CD4+ cells in resistance to Pneumocystis carinii pneumonia in mice.
Requirement for CD4+ cells in resistance to Pneumocystis carinii pneumonia in mice.
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DOI：
10.1084/jem.172.3.937
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发表时间：
1990-09-01
影响因子：
15.3
通讯作者：
Stankiewicz, M
中科院分区：
文献类型：
--
作者：
Harmsen, A G;Stankiewicz, M
通过免疫活性脾细胞转移对卡氏肺孢子虫（PC）敏感的严重联合免疫缺陷（SCID）小鼠和常规PC耐药小鼠中的CD 4+细胞在PC耐药中的重要性进行了研究。对患有自然获得性PC肺炎（PCP）的SCID小鼠输注免疫活性供体的脾细胞。这种重组使受体解决了他们的PCP。用抗CD 4单克隆抗体（mAb）处理重建的SCID小鼠以耗尽其CD 4+细胞，消除了其分解PCP的能力，而用抗CD 8 mAb处理以耗尽CD 8+细胞则没有效果。因此，研究结果表明，免疫重建的SCID小鼠对PCP的抵抗力取决于CD 4+细胞。为了确定CD 4+细胞是否能够使常规小鼠抵抗PCP，用抗CD 4 mAb处理B6 D2小鼠以消耗它们的CD 4+细胞，试图诱导PCP。治疗10-11周后，这些小鼠发生进行性PCP。总之，这些结果表明，CD 4+细胞的损失使小鼠易于PC感染。
The importance of CD4+ cells in resistance to Pneumocystis carinii (PC) in PC-susceptible severe combined immunodeficient (SCID) mice that were made resistant to PC by immunocompetent spleen cell transfer, and in conventional PC-resistant mice, was investigated. SCID mice with naturally acquired PC pneumonia (PCP) were given infusions of spleen cells from immunocompetent donors. This reconstitution caused the recipients to resolve their PCP. Treatment of reconstituted SCID mice with anti-CD4 monoclonal antibodies (mAbs) to deplete them of CD4+ cells eliminated their ability to resolve PCP, whereas treating them with anti-CD8 mAb to deplete CD8+ cells had no effect. The findings indicate, therefore, that resistance to PCP in immunologically reconstituted SCID mice is dependent on CD4+ cells. To determine whether CD4+ cells enable conventional mice to resist PCP, B6D2 mice were treated with anti-CD4 mAb to deplete them of CD4+ cells in an attempt to induce PCP. After 10-11 wk of treatment, these mice developed progressive PCP. Taken together, these results indicate that loss of CD4+ cells predisposes mice to PC infection.