SEROLOGICAL DIAGNOSIS OF INFLUENZA B-VIRUS INFECTION - COMPARISON OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY AND THE HEMAGGLUTINATION INHIBITION TEST
SEROLOGICAL DIAGNOSIS OF INFLUENZA B-VIRUS INFECTION - COMPARISON OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY AND THE HEMAGGLUTINATION INHIBITION TEST
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DOI：
10.1128/jcm.15.5.824-829.1982
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发表时间：
1982-01-01
影响因子：
9.4
通讯作者：
BELSHE, RB
中科院分区：
文献类型：
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作者：
TURNER, R;LATHEY, JL;BELSHE, RB
用酶联免疫吸附试验（ELISA）检测乙型流感病毒抗体的敏感性与血凝抑制试验对乙型流感病毒感染成人和儿童急性期和恢复期血清的敏感性进行了比较。ELISA采用了两种全病毒、组织培养抗原制剂：流感B/West Virginia/81和流感B/Hong Kong/72。四种抗原用于血凝抑制试验。这些包括在ELISA中使用的组织培养的全病毒抗原。采用标准的蛋培养抗原B型流感/新加坡/79和B型流感/香港/72进行比较。ELISA抗体滴度与血凝抑制滴度显著相关；通过ELISA检测，10名成人和21名儿童感染乙型流感病毒时，抗原型组织培养全病毒中有10人抗体升高4倍。ELISA检测到几何平均抗体滴度增加16 ~ 71倍。几何平均抗体滴度的3- 10倍的增加检测到血凝抑制取决于所用抗原的类型。可见，全病毒抗原ELISA检测乙型流感病毒抗体是一种灵敏、特异的检测方法。
The sensitivity of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to influenza B virus was compared with that of the hemagglutination inhibition test on acute- and convalescent-phase sera obtained from adults and children infected with influenza B virus. Two whole virus, tissue culture-grown antigen preparations were used in the ELISA, influenza B/West Virginia/81 and influenza B/Hong Kong/72. Four antigens were used in the hemagglutination inhibition test. These included the tissue culture-grown whole virus antigens that were used in the ELISA. The standard egg-grown antigens, influenza B/Singapore/79 and influenza B/Hong Kong/72, were included for comparison. The ELISA antibody titer was significantly correlated to the hemagglutination inhibition titer; 10 of 10 adults and 17 of 21 children infected with influenza B had 4-fold antibody increases as detected by ELISA with either antigenic type of tissue culture-grown whole virus. Increases in geometric mean antibody titers of 16- to 71-fold were detected by ELISA. Increases in geometric mean antibody titers of 3- to 10-fold were detected by hemagglutination inhibition depending on the type of antigen utilized. Evidently, ELISA with whole virus antigens was a sensitive and specific test for the detection of antibody to influenza B virus.