Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
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DOI：
10.1136/gut.2010.225268
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发表时间：
2011-07-01
期刊：
影响因子：
24.5
通讯作者：
Sousa, Carolina
中科院分区：
文献类型：
--
作者：
Comino, Isabel;Real, Ana;Sousa, Carolina
背景和目的乳糜泻（CD）是由对谷蛋白的异常反应引起的。由小麦、大麦或黑麦部分消化的麸质产生的肽可引起小肠黏膜炎症。以往相互矛盾的研究表明燕麦可能引发CD患者的异常免疫反应。针对主要免疫毒性33-mer肽（A1和G12）的单克隆抗体（moAbs）对小麦、大麦和黑麦反应强烈，但对燕麦反应较弱。本研究的目的是检验这种反应性是否与燕麦对cd患者的潜在毒性有关。方法本研究采用不同燕麦品种，控制其纯度和不同的蛋白质模式，通过ELISA和western blot检测moAb G12识别的差异。免疫原性通过33-mer浓度、T细胞增殖和干扰素γ的产生来确定。结果不同燕麦品种对moabag12的亲和程度有明显差异，对moabag12有轻微反应，对moabag12无明显反应。利用分离的CD患者外周血单个核T细胞，通过测量细胞增殖和干扰素γ释放来测定三种燕麦的免疫原性以及阳性和阴性对照。不同蛋白的免疫原性与G12的反应性直接相关。结论mababg12的反应性与潜在的免疫毒性成正比。这些差异可能解释了在乳糜泻患者中观察到的不同临床反应，并为鉴定免疫安全的燕麦品种开辟了一条途径，可用于丰富无麸质饮食。
Background and aims Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunological response in patients with CD. Monoclonal antibodies (moAbs) against the main immunotoxic 33-mer peptide (A1 and G12) react strongly against wheat, barley and rye but have less reactivity against oats. The stated aim of this study is to test whether this observed reactivity could be related to the potential toxicity of oats for patients with CD.Methods In the present study, different oat varieties, controlled for their purity and by their distinct protein pattern, were used to examine differences in moAb G12 recognition by ELISA and western blot. Immunogenicity of oat varieties was determined by 33-mer concentration, T cell proliferation and interferon gamma production.Results Three groups of oat cultivars reacting differently against moAb G12 could be distinguished: a group with considerable affinity, a group showing slight reactivity and a third with no detectable reactivity. The immunogenicity of the three types of oats as well as that of a positive and negative control was determined with isolated peripheral blood mononuclear T cells from patients with CD by measurement of cell proliferation and interferon gamma release. A direct correlation of the reactivity with G12 and the immunogenicity of the different prolamins was observed.Conclusions The results showed that the reactivity of the moAb G12 is proportional to the potential immunotoxicity of the cereal cultivar. These differences may explain the different clinical responses observed in patients suffering from CD and open up a means to identify immunologically safe oat cultivars, which could be used to enrich a gluten-free diet.