Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract
Ribonuclease 7 is a potent antimicrobial peptide within the human urinary tract
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DOI：
10.1038/ki.2011.109
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发表时间：
2011-07-01
影响因子：
19.6
通讯作者：
Hains, David S.
中科院分区：
文献类型：
--
作者：
Spencer, John D.;Schwaderer, Andrew L.;Hains, David S.
虽然尿路不断受到微生物入侵的挑战，但它仍然没有定植。虽然对尿路如何保持无菌性知之甚少，但尿液中抗菌肽（AMP）的存在表明它们可能在保护其免受感染方面发挥作用。核糖核酸酶7（RNase 7）是一种有效的AMP，首先在皮肤中发现。在这里，我们描述了RNase 7在人类肾脏和泌尿道中的表达和相关性。使用从健康人体组织中分离的RNA，我们进行了定量实时PCR，并发现肾脏和膀胱组织中的基础RNASE 7表达。免疫组织化学和免疫荧光分析定位RNase 7的膀胱，输尿管，和集合管的闰细胞的尿道。在来自健康个体的对照尿样中，发现RNase 7的浓度在低微摩尔范围内;对于AMP来说非常丰富。抗菌中和试验表明，尿RNase 7对革兰氏阴性和革兰氏阳性尿路致病菌具有有效的抗菌特性。因此，RNase 7在人的肾脏和泌尿道中表达，并且它可能在维持泌尿道无菌性方面具有重要的抗微生物作用。Kidney International（2011）80，174-180; doi：10.1038/ki.2011.109; 2011年4月27日在线发表
Although the urinary tract is constantly challenged by microbial invasion, it remains free from colonization. Although little is known about how the urinary tract maintains sterility, the presence of antimicrobial peptides (AMPs) in the urine suggests that they may play a role in its protection from infection. Ribonuclease 7 (RNase 7) is a potent AMP that was first identified in the skin. Here, we characterize the expression and relevance of RNase 7 in the human kidney and urinary tract. Using RNA isolated from healthy human tissue, we performed quantitative real-time PCR and found basal RNASE7 expression in kidney and bladder tissue. Immunohistochemical and immunofluorescent analysis localized RNase 7 to the urothelium of the bladder, ureter, and the intercalated cells of the collecting tubules. In control urine samples from healthy individuals, the concentration of RNase 7 was found to be in the low micromolar range; very abundant for an AMP. Antibacterial neutralization assays showed that urinary RNase 7 has potent antimicrobial properties against Gram-negative and Gram-positive uropathogenic bacteria. Thus, RNase 7 is expressed in the human kidney and urinary tract and it may have an important antimicrobial role in maintaining tract sterility. Kidney International (2011) 80, 174-180; doi:10.1038/ki.2011.109; published online 27 April 2011