Spatial and temporal evolution of neuronal activation, stress and injury in lithium-pilocarpine seizures in adult rats
Spatial and temporal evolution of neuronal activation, stress and injury in lithium-pilocarpine seizures in adult rats
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DOI：
10.1016/s0006-8993(98)00135-8
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发表时间：
1998-05-18
期刊：
影响因子：
2.9
通讯作者：
Nehlig, A
中科院分区：
文献类型：
--
作者：
Motte, J;Fernandes, MJD;Nehlig, A
为了跟踪成年大鼠不同持续时间的锂毛果芸香碱癫痫发作后神经元损伤、细胞激活和应激反应的空间和时间演变，我们分析了 Fos 蛋白的表达和局部脑葡萄糖利用作为细胞激活的标记，HSP72 免疫反应性和酸性品红染色作为细胞应激和损伤的指标，以及甲酚紫染色用于评估神经元损伤。 Fos 的表达很早就出现，即多刺出现后 2-30 分钟，并在接下来的 4 小时内增强。 Fos 免疫反应性在海马、大脑皮层、杏仁核和前嗅核中尤其高。在癫痫发作的第二个小时内测得的局部大脑葡萄糖利用率比皮质区、杏仁核、齿状回、尾状核和内侧丘脑的对照水平大幅增加（350-580%）。 HSP72免疫反应性在多尖峰出现后40-50分钟内从未出现过，并且在海马CA3区、大脑皮层（梨状皮层除外）和前嗅核中最为突出。酸性品红染色在梨状皮层和齿状回的多态层中最多。感觉运动皮层和杏仁核染色程度中等。梨状肌神经元损伤广泛。内嗅皮质、海马 CA3 区以及齿状回、基底杏仁核、内侧丘脑和前嗅核的多态层。总之，本研究表明 Fos 表达最高和代谢激活最大的大脑区域也被酸性品红高度染色且受损最严重。相反，HSP72 表达、细胞激活和神经元损伤之间没有明确的关系。 (C) 1998 Elsevier Science B.V. 保留所有权利。
In order to follow the spatial and temporal evolution of neuronal damage, cellular activation and stress responses subsequent to lithium-pilocarpine seizures of various durations in the adult rat, we analyzed the expression of Fos protein and local cerebral glucose utilization as markers of cellular activation, HSP72 immunoreactivity and acid fuchsin staining as indicators of cellular stress and injury, and Cresyl violet staining for the assessment of neuronal damage. The expression of Fos appeared very early, 2-30 min after the onset of polyspikes and intensified during the following 4 h. Fos immunoreactivity was especially high in the hippocampus, cerebral cortex, amygdala and anterior olfactory nuclei. Local cerebral glucose utilization measured during the second hour of seizures was largely increased (350-580%) over control levels in cortical areas, amygdala, dentate gyrus, caudate nucleus and mediodorsal thalamus. HSP72 immunoreactivity never appeared earlier than 40-50 min after the onset of polyspikes, and was most prominent in hippocampal CA3 area, cerebral cortex (except the piriform cortex) and anterior olfactory nuclei. Acid fuchsin staining was maximal in the piriform cortex and the polymorphic layer of the dentate gyrus. Staining was moderate in the sensorimotor cortex and the amygdala. Neuronal damage was extensive in the piriform. and entorhinal cortices, the hippocampal CA3 area and the polymorphic layer of the dentate gyrus, basal amygdala, mediodorsal thalamus and anterior olfactory nuclei. In conclusion, the present study shows that brain regions with the highest expression of Fos and the largest metabolic activation were also highly stained with acid fuchsin and most heavily damaged. Conversely, there is no clear relationship between HSP72 expression, cellular activation and neuronal damage. (C) 1998 Elsevier Science B.V. All rights reserved.